Detailed information of TA system TA065752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2867431..2868071
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BB988_RS13995
• Protein ID: WP_003412970.1
• Coordinates: 2867431..2867850 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BB988_RS14000
• Protein ID: WP_003412975.1
• Coordinates: 2867847..2868071 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS13965	2863016..2863738	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
BB988_RS13975	2864255..2864482	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
BB988_RS13980	2864479..2864880	+	402	WP_003412963.1	PIN domain-containing protein	-
BB988_RS13985	2864915..2865835	-	921	WP_003412965.1	restriction endonuclease	-
BB988_RS22350	2866176..2866421	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BB988_RS13990	2866480..2867430	+	951	WP_003911916.1	Lsr2 family protein	-
BB988_RS13995	2867431..2867850	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB988_RS14000	2867847..2868071	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BB988_RS14005	2868102..2870945	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BB988_RS14010	2871017..2871418	-	402	WP_003412981.1	hypothetical protein	-
BB988_RS14015	2871418..2871888	-	471	WP_003899364.1	transcription antitermination factor NusB	-
BB988_RS14020	2871891..2872454	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T65752 WP_003412970.1 NZ_CP016972:c2867850-2867431 [Mycobacterium tuberculosis H37Ra]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T65752 NZ_CP016972:c2867850-2867431 [Mycobacterium tuberculosis H37Ra]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT65752 WP_003412975.1 NZ_CP016972:c2868071-2867847 [Mycobacterium tuberculosis H37Ra]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT65752 NZ_CP016972:c2868071-2867847 [Mycobacterium tuberculosis H37Ra]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09