Detailed information of TA system TA065746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2269143..2269769
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BB988_RS11035
• Protein ID: WP_003410075.1
• Coordinates: 2269371..2269769 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: BB988_RS11030
• Protein ID: WP_003911750.1
• Coordinates: 2269143..2269370 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS11020	2267182..2267526	-	345	WP_003410065.1	ferredoxin family protein	-
BB988_RS11025	2267715..2268884	-	1170	WP_003899126.1	ATP-binding protein	-
BB988_RS11030	2269143..2269370	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BB988_RS11035	2269371..2269769	+	399	WP_003410075.1	PIN domain nuclease	Toxin
BB988_RS11040	2269952..2270383	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BB988_RS11045	2270484..2270918	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
BB988_RS21910	2271355..2271534	-	180	Protein_2092	hypothetical protein	-
BB988_RS11065	2271595..2272242	+	648	WP_075631456.1	transposase	-
BB988_RS11070	2272196..2272786	+	591	WP_003899131.1	IS110 family transposase	-
BB988_RS11075	2272914..2274170	-	1257	WP_003410095.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T65746 WP_003410075.1 NZ_CP016972:2269371-2269769 [Mycobacterium tuberculosis H37Ra]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T65746 NZ_CP016972:2269371-2269769 [Mycobacterium tuberculosis H37Ra]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT65746 WP_003911750.1 NZ_CP016972:2269143-2269370 [Mycobacterium tuberculosis H37Ra]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT65746 NZ_CP016972:2269143-2269370 [Mycobacterium tuberculosis H37Ra]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8