Detailed information of TA system TA065740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2205741..2206444
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BB988_RS10645
• Protein ID: WP_003409778.1
• Coordinates: 2205741..2206070 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BB988_RS10650
• Protein ID: WP_003409780.1
• Coordinates: 2206067..2206444 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS10625	2202124..2203194	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
BB988_RS10630	2203191..2203706	+	516	WP_003409718.1	flavin reductase family protein	-
BB988_RS10635	2203703..2204764	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BB988_RS10640	2204761..2205531	+	771	WP_003409775.1	SDR family oxidoreductase	-
BB988_RS10645	2205741..2206070	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BB988_RS10650	2206067..2206444	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BB988_RS10655	2206441..2207031	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BB988_RS10660	2207086..2208450	+	1365	WP_003903691.1	HNH endonuclease	-
BB988_RS10665	2208605..2209057	-	453	WP_003899095.1	lipoprotein	-
BB988_RS10670	2209121..2209522	+	402	WP_003409869.1	hypothetical protein	-
BB988_RS10675	2209515..2209697	-	183	WP_003409870.1	hypothetical protein	-
BB988_RS10680	2209811..2210161	-	351	WP_003899096.1	hypothetical protein	-
BB988_RS10685	2210172..2211074	-	903	WP_003899097.1	hypothetical protein	-
BB988_RS10690	2211095..2211286	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T65740 WP_003409778.1 NZ_CP016972:c2206070-2205741 [Mycobacterium tuberculosis H37Ra]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T65740 NZ_CP016972:c2206070-2205741 [Mycobacterium tuberculosis H37Ra]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT65740 WP_003409780.1 NZ_CP016972:c2206444-2206067 [Mycobacterium tuberculosis H37Ra]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT65740 NZ_CP016972:c2206444-2206067 [Mycobacterium tuberculosis H37Ra]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8