Detailed information of TA system TA065737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1766341..1766954
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: BB988_RS08570
• Protein ID: WP_003407786.1
• Coordinates: 1766550..1766954 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: BB988_RS08565
• Protein ID: WP_009935474.1
• Coordinates: 1766341..1766544 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS08535	1761746..1762126	+	381	WP_003916859.1	fumarate reductase subunit C	-
BB988_RS08540	1762123..1762500	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
BB988_RS08545	1762568..1763176	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
BB988_RS08550	1763360..1764508	+	1149	Protein_1616	MMPL family transporter	-
BB988_RS08555	1764518..1764964	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
BB988_RS08560	1764999..1766288	+	1290	WP_003407781.1	threonine ammonia-lyase	-
BB988_RS08565	1766341..1766544	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BB988_RS08570	1766550..1766954	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
BB988_RS08575	1766971..1768713	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
BB988_RS08580	1768706..1771003	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T65737 WP_003407786.1 NZ_CP016972:1766550-1766954 [Mycobacterium tuberculosis H37Ra]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T65737 NZ_CP016972:1766550-1766954 [Mycobacterium tuberculosis H37Ra]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT65737 WP_009935474.1 NZ_CP016972:1766341-1766544 [Mycobacterium tuberculosis H37Ra]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT65737 NZ_CP016972:1766341-1766544 [Mycobacterium tuberculosis H37Ra]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46