Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1687842..1688458 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag BB988_RS08215 Protein ID WP_003407593.1
Coordinates 1688141..1688458 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag BB988_RS08210 Protein ID WP_003900349.1
Coordinates 1687842..1688144 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS08200 1683728..1685575 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
BB988_RS08205 1685576..1687828 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
BB988_RS08210 1687842..1688144 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
BB988_RS08215 1688141..1688458 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
BB988_RS08220 1688455..1689459 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
BB988_RS08225 1689512..1690801 + 1290 WP_003407599.1 serine hydrolase -
BB988_RS08230 1690874..1691647 - 774 WP_003911559.1 class I SAM-dependent methyltransferase -
BB988_RS08235 1691705..1691917 - 213 WP_003898900.1 dodecin family protein -
BB988_RS22220 1691927..1692151 - 225 WP_160522526.1 NUDIX domain-containing protein -
BB988_RS08245 1692421..1693449 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T65736 WP_003407593.1 NZ_CP016972:1688141-1688458 [Mycobacterium tuberculosis H37Ra]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T65736 NZ_CP016972:1688141-1688458 [Mycobacterium tuberculosis H37Ra]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT65736 WP_003900349.1 NZ_CP016972:1687842-1688144 [Mycobacterium tuberculosis H37Ra]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT65736 NZ_CP016972:1687842-1688144 [Mycobacterium tuberculosis H37Ra]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References