Detailed information of TA system TA065731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1240779..1241347
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BB988_RS06125
• Protein ID: WP_003405865.1
• Coordinates: 1240973..1241347 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BB988_RS06120
• Protein ID: WP_003405863.1
• Coordinates: 1240779..1240976 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS06100	1236820..1237458	-	639	WP_003898730.1	lipid droplet-associated protein	-
BB988_RS06105	1237548..1238555	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BB988_RS06110	1238572..1239555	-	984	WP_003898731.1	hypothetical protein	-
BB988_RS06115	1239618..1240691	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BB988_RS06120	1240779..1240976	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BB988_RS06125	1240973..1241347	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BB988_RS06130	1241550..1242248	+	699	WP_003898733.1	hypothetical protein	-
BB988_RS06135	1242366..1242551	+	186	WP_003901093.1	hypothetical protein	-
BB988_RS06140	1242478..1242753	-	276	WP_003405867.1	hypothetical protein	-
BB988_RS06145	1242996..1243319	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BB988_RS06150	1243334..1244044	-	711	WP_003911399.1	hypothetical protein	-
BB988_RS22180	1244066..1244275	+	210	WP_003911400.1	hypothetical protein	-
BB988_RS06160	1244227..1244934	-	708	Protein_1163	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T65731 WP_003405865.1 NZ_CP016972:1240973-1241347 [Mycobacterium tuberculosis H37Ra]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T65731 NZ_CP016972:1240973-1241347 [Mycobacterium tuberculosis H37Ra]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT65731 WP_003405863.1 NZ_CP016972:1240779-1240976 [Mycobacterium tuberculosis H37Ra]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT65731 NZ_CP016972:1240779-1240976 [Mycobacterium tuberculosis H37Ra]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07