Detailed information of TA system TA065714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 641592..642268
• Replicon: chromosome
• Accession: NZ_CP016972
• Organism: Mycobacterium tuberculosis H37Ra

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: BB988_RS02940
• Protein ID: WP_003898500.1
• Coordinates: 641592..642005 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: BB988_RS02945
• Protein ID: WP_003402915.1
• Coordinates: 642002..642268 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB988_RS02910	636937..637239	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
BB988_RS02915	637299..637577	-	279	WP_003402901.1	hypothetical protein	-
BB988_RS02920	637574..638827	-	1254	WP_003911224.1	inorganic phosphate transporter family protein	-
BB988_RS02925	638947..639333	-	387	WP_003402907.1	VOC family protein	-
BB988_RS02930	639396..640280	-	885	WP_003402909.1	SDR family oxidoreductase	-
BB988_RS02935	640376..641278	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
BB988_RS02940	641592..642005	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
BB988_RS02945	642002..642268	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
BB988_RS02950	642460..644175	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
BB988_RS02955	644253..645857	+	1605	WP_003402920.1	amidohydrolase	-
BB988_RS02960	645854..646834	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T65714 WP_003898500.1 NZ_CP016972:c642005-641592 [Mycobacterium tuberculosis H37Ra]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T65714 NZ_CP016972:c642005-641592 [Mycobacterium tuberculosis H37Ra]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT65714 WP_003402915.1 NZ_CP016972:c642268-642002 [Mycobacterium tuberculosis H37Ra]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT65714 NZ_CP016972:c642268-642002 [Mycobacterium tuberculosis H37Ra]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0