65714

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 641592..642268 Replicon chromosome
Accession NZ_CP016972
Organism Mycobacterium tuberculosis H37Ra

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag BB988_RS02940 Protein ID WP_003898500.1
Coordinates 641592..642005 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag BB988_RS02945 Protein ID WP_003402915.1
Coordinates 642002..642268 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BB988_RS02910 636937..637239 - 303 WP_003402896.1 DUF3349 domain-containing protein -
BB988_RS02915 637299..637577 - 279 WP_003402901.1 hypothetical protein -
BB988_RS02920 637574..638827 - 1254 WP_003911224.1 inorganic phosphate transporter family protein -
BB988_RS02925 638947..639333 - 387 WP_003402907.1 VOC family protein -
BB988_RS02930 639396..640280 - 885 WP_003402909.1 SDR family oxidoreductase -
BB988_RS02935 640376..641278 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
BB988_RS02940 641592..642005 - 414 WP_003898500.1 PIN domain-containing protein Toxin
BB988_RS02945 642002..642268 - 267 WP_003402915.1 antitoxin VapB3 Antitoxin
BB988_RS02950 642460..644175 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
BB988_RS02955 644253..645857 + 1605 WP_003402920.1 amidohydrolase -
BB988_RS02960 645854..646834 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T65714 WP_003898500.1 NZ_CP016972:c642005-641592 [Mycobacterium tuberculosis H37Ra]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T65714 NZ_CP016972:c642005-641592 [Mycobacterium tuberculosis H37Ra]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT65714 WP_003402915.1 NZ_CP016972:c642268-642002 [Mycobacterium tuberculosis H37Ra]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT65714 NZ_CP016972:c642268-642002 [Mycobacterium tuberculosis H37Ra]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References