Detailed information of TA system TA065681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 6141456..6142877
• Replicon: chromosome
• Accession: NZ_CP016955
• Organism: Pseudomonas aeruginosa strain RIVM-EMC2982

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: BFV99_RS29605
• Protein ID: WP_025297643.1
• Coordinates: 6141456..6142529 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: BFV99_RS29610
• Protein ID: WP_158025315.1
• Coordinates: 6142584..6142877 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV99_RS29580	6137549..6137977	-	429	WP_025297641.1	S24 family peptidase	-
BFV99_RS29585	6138257..6139840	+	1584	WP_025297642.1	hypothetical protein	-
BFV99_RS29590	6139893..6140189	-	297	WP_023128612.1	hypothetical protein	-
BFV99_RS29600	6140487..6140741	-	255	WP_023128610.1	hypothetical protein	-
BFV99_RS29605	6141456..6142529	+	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
BFV99_RS29610	6142584..6142877	+	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
BFV99_RS29615	6143016..6143809	-	794	Protein_5737	transporter substrate-binding domain-containing protein	-
BFV99_RS29620	6143784..6143978	+	195	WP_023128607.1	OprD family outer membrane porin	-
BFV99_RS29625	6144202..6144566	-	365	Protein_5739	DNA binding protein	-
BFV99_RS29630	6144897..6145475	-	579	WP_025297644.1	hypothetical protein	-
BFV99_RS29635	6145557..6145940	-	384	WP_025297645.1	hypothetical protein	-
BFV99_RS29640	6145943..6146413	-	471	WP_023128604.1	hypothetical protein	-
BFV99_RS29645	6146456..6146851	-	396	WP_023128603.1	hypothetical protein	-
BFV99_RS29650	6147016..6147471	+	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
BFV99_RS29655	6147562..6147774	-	213	WP_023128602.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6084607..6156147	71540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T65681 WP_025297643.1 NZ_CP016955:6141456-6142529 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T65681 NZ_CP016955:6141456-6142529 [Pseudomonas aeruginosa]
GTGACTGAACGCGACTACAAAAACATCAACCTCAAACGATGCATTATCGAGGAAACGACTCATTGCTTCATCCGAGATAA
TGGCCTGAAGCTGCGATCCTATGGTGACAAGCCTGGGAGTTCCGGTCTTCGAGTAGTCTTCGGAAAAGCAGGGATTGAAG
ATGCCACGCTTGATATTTTTTTCACGAATGTCGGTGCGTCTACCCTGACCTACAAGGTGGGCAAGAACCAAGCTCTCGGC
CAGCAGCTAGCGGATGCTCTTTACGAAACAATCCACCCCGATGAATTCGTAACGATGAACCTTGGTATCAAAGGCGTCGA
TCTGGATGATGCCGAAGCCCTTATCAAGGAACTCACTGAGTCAGCCAATGCGGACATTGAGGTTGCTTCGCGCACAAGCG
CTGATCGCAAGCATATTTGGAAGCTCAAGTCTAAAAAGAACTTGGATGAGTTGACTGTCACTCAGCACGAGAACACCCAT
AAACTGCAGATCCAGGGTCGTCCTCTCAGCTGCTATCAGCAGTTGAGCTACCTGCTCACCGAGCTCCTAGAGATCAACGC
ACTCGAATGCATCCTGTTCAAAAAGGAAGAAAATCGCTCGGAGTTCGTTTGTGCCGAGGTCGCGGAAAGCGTACTCAAGG
AAAGGTTTGGCGACTGCTACGCCAACCTGCCACAGACCTCGCGTAGGCTGCTCCTGGCAAGCCTTTGCGTGAGGCTTGCC
AGCCCCGCGCTACCCGACTACTGCATGCTGGTTTATCCGGAACTCCGCACCCTTGAAGGCGCGATCAAACAGAAGCTTGC
AGAAAAAAACCTTTCAAATCAGGAAGAAAGCTTCGGTGGCTTCTTTACCAAGACCAATGGTCAGTTTTATCTGAAGCAGC
AATACCATGCTGATGTCGGCGATGCTAAACTACAGGGTCATCTGGACAACGCTTACACCTTCTTCAACAAGCAACGCCAC
GGATTGTTCCACATGGAAGAGATGCCATCCGTTAGTCGAATGATTAGCAACATCACCCAAGCTGTGTCATTGTGTGATGA
AGCATATGATCATATCAAGAATTTGTATAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT65681 WP_158025315.1 NZ_CP016955:6142584-6142877 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT65681 NZ_CP016955:6142584-6142877 [Pseudomonas aeruginosa]
ATGACGGTAGTTACCGCTACCAGTTATGAGAACCCCTTATCTCGTCTTTCTATTATTGCTAGCGAGATGCGCAATACATC
TCACTCCAGCAAGGAGATAGTTCTATTCGATCTACTCTGCTCTAACGGAGAAGAGTGGAATCGTTTCGTCTCCATCAATT
ACAACGGCACCGACTTCGAAAAAAGCACCTGTAGCATTGTCTCCAAGTCCGACATCCCTACAGACCTCTTGGAAACACAA
ACTCGCTTCTTTCAAATCCATCCACAATATCTGCTTGATTCAGTTTTGAATTAA