Detailed information of TA system TA065679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4626987..4628029
• Replicon: chromosome
• Accession: NZ_CP016955
• Organism: Pseudomonas aeruginosa strain RIVM-EMC2982

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BFV99_RS22160
• Protein ID: WP_003153636.1
• Coordinates: 4626987..4627562 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: BFV99_RS22165
• Protein ID: WP_003050245.1
• Coordinates: 4627559..4628029 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFV99_RS22135	4623435..4624160	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
BFV99_RS22140	4624199..4625101	-	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
BFV99_RS22145	4625101..4625591	-	491	Protein_4282	HORMA domain containing protein	-
BFV99_RS22150	4625588..4626058	-	471	WP_003090160.1	hypothetical protein	-
BFV99_RS22155	4626055..4626969	-	915	WP_016852809.1	AAA family ATPase	-
BFV99_RS22160	4626987..4627562	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
BFV99_RS22165	4627559..4628029	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
BFV99_RS22170	4628233..4628616	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
BFV99_RS22175	4628613..4628846	+	234	WP_023101201.1	TIGR03758 family integrating conjugative element protein	-
BFV99_RS22180	4628863..4629222	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
BFV99_RS22185	4629234..4629632	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
BFV99_RS22190	4629629..4630321	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
BFV99_RS22195	4630318..4631229	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
BFV99_RS22200	4631219..4632637	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4581504..4704966	123462

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T65679 WP_003153636.1 NZ_CP016955:c4627562-4626987 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T65679 NZ_CP016955:c4627562-4626987 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT65679 WP_003050245.1 NZ_CP016955:c4628029-4627559 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT65679 NZ_CP016955:c4628029-4627559 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68