Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4778476..4779096 Replicon chromosome
Accession NZ_CP016952
Organism Citrobacter freundii strain SL151

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BFQ28_RS22605 Protein ID WP_002892050.1
Coordinates 4778878..4779096 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag BFQ28_RS22600 Protein ID WP_003021733.1
Coordinates 4778476..4778850 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFQ28_RS22590 4773622..4774815 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BFQ28_RS22595 4774838..4777987 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
BFQ28_RS22600 4778476..4778850 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
BFQ28_RS22605 4778878..4779096 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BFQ28_RS22610 4779403..4779954 + 552 WP_003835924.1 maltose O-acetyltransferase -
BFQ28_RS22615 4780071..4780541 + 471 WP_003021724.1 YlaC family protein -
BFQ28_RS22620 4780620..4780760 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
BFQ28_RS22625 4780762..4781022 - 261 WP_003835926.1 type B 50S ribosomal protein L31 -
BFQ28_RS22630 4781211..4782764 + 1554 WP_046669815.1 EAL domain-containing protein -
BFQ28_RS22635 4782816..4783169 - 354 WP_003831033.1 DUF1428 family protein -
BFQ28_RS22640 4783234..4783863 - 630 WP_016149709.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T65670 WP_002892050.1 NZ_CP016952:4778878-4779096 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T65670 NZ_CP016952:4778878-4779096 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT65670 WP_003021733.1 NZ_CP016952:4778476-4778850 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT65670 NZ_CP016952:4778476-4778850 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References