Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4113629..4114219 Replicon chromosome
Accession NZ_CP016952
Organism Citrobacter freundii strain SL151

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag BFQ28_RS19510 Protein ID WP_003836692.1
Coordinates 4113629..4113961 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag BFQ28_RS19515 Protein ID WP_003836694.1
Coordinates 4113962..4114219 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFQ28_RS19485 4109382..4110869 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
BFQ28_RS19495 4111185..4112135 - 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
BFQ28_RS19500 4112237..4113154 - 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
BFQ28_RS19510 4113629..4113961 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BFQ28_RS19515 4113962..4114219 - 258 WP_003836694.1 hypothetical protein Antitoxin
BFQ28_RS19525 4114833..4118228 + 3396 Protein_3831 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4103244..4135417 32173


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T65669 WP_003836692.1 NZ_CP016952:c4113961-4113629 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T65669 NZ_CP016952:c4113961-4113629 [Citrobacter freundii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCAGCATCATTTAATAAGTTCACCCGGCTACCTGTTGTCGTTCCCGTCACCAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCTCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTTGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT65669 WP_003836694.1 NZ_CP016952:c4114219-4113962 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT65669 NZ_CP016952:c4114219-4113962 [Citrobacter freundii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References