Detailed information of TA system TA065668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3520641..3521280
• Replicon: chromosome
• Accession: NZ_CP016952
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: BFQ28_RS27260
• Protein ID: WP_003020221.1
• Coordinates: 3520641..3520817 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BFQ28_RS16670
• Protein ID: WP_123924900.1
• Coordinates: 3520864..3521280 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS16650	3517419..3517649	-	231	WP_046670718.1	DUF2554 family protein	-
BFQ28_RS16655	3517839..3517964	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
BFQ28_RS16660	3517964..3518974	-	1011	WP_046670717.1	cytochrome d ubiquinol oxidase subunit II	-
BFQ28_RS16665	3518974..3520377	-	1404	WP_003843730.1	cytochrome ubiquinol oxidase subunit I	-
BFQ28_RS27260	3520641..3520817	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
BFQ28_RS16670	3520864..3521280	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BFQ28_RS16675	3521373..3522782	+	1410	WP_046670716.1	PLP-dependent aminotransferase family protein	-
BFQ28_RS16680	3523111..3524256	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
BFQ28_RS16685	3524273..3525289	+	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
BFQ28_RS16690	3525290..3526234	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T65668 WP_003020221.1 NZ_CP016952:3520641-3520817 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T65668 NZ_CP016952:3520641-3520817 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT65668 WP_123924900.1 NZ_CP016952:3520864-3521280 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT65668 NZ_CP016952:3520864-3521280 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCGGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36