Detailed information of TA system TA065659

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1976598..1977259
• Replicon: chromosome
• Accession: NZ_CP016952
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: BFQ28_RS09465
• Protein ID: WP_048997879.1
• Coordinates: 1976936..1977259 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A241Q747
• Locus tag: BFQ28_RS09460
• Protein ID: WP_048997880.1
• Coordinates: 1976598..1976915 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS09420	1972589..1973299	+	711	WP_048998192.1	DeoR family transcriptional regulator	-
BFQ28_RS09425	1973325..1973861	+	537	WP_048998191.1	DUF4339 domain-containing protein	-
BFQ28_RS09430	1973913..1974479	+	567	WP_048998190.1	hypothetical protein	-
BFQ28_RS09435	1974569..1974979	+	411	WP_048998189.1	hypothetical protein	-
BFQ28_RS09440	1975057..1975296	+	240	WP_048998188.1	DUF905 domain-containing protein	-
BFQ28_RS09445	1975399..1975857	+	459	WP_069219491.1	antirestriction protein	-
BFQ28_RS09450	1975873..1976349	+	477	WP_049016517.1	RadC family protein	-
BFQ28_RS09455	1976358..1976579	+	222	WP_048997881.1	DUF987 domain-containing protein	-
BFQ28_RS09460	1976598..1976915	+	318	WP_048997880.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFQ28_RS09465	1976936..1977259	+	324	WP_048997879.1	TA system toxin CbtA family protein	Toxin
BFQ28_RS09470	1977499..1978035	-	537	WP_032949993.1	GNAT family N-acetyltransferase	-
BFQ28_RS09475	1978852..1981318	+	2467	Protein_1834	poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1945625..1977259	31634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12455.32 Da
• Isoelectric Point: 6.4624

>T65659 WP_048997879.1 NZ_CP016952:1976936-1977259 [Citrobacter freundii]
MKSQPATTQRAAKPCLSPVDVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLLNRN

Nucleotide

• Length: 324 bp

>T65659 NZ_CP016952:1976936-1977259 [Citrobacter freundii]
ATGAAATCTCAACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGATGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCATTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGTATCACGCTGGCTGATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGATTATTGATATCATGAGGGCCCGCCGGGATCTGGGTTTATTAAATCGTAA
TTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11993.64 Da
• Isoelectric Point: 7.6976

>AT65659 WP_048997880.1 NZ_CP016952:1976598-1976915 [Citrobacter freundii]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGQFSDAECRKLDVAFPYFIRQMESMLTTGELNPRHTHCVTLYR
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT65659 NZ_CP016952:1976598-1976915 [Citrobacter freundii]
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATTACGCCACGCCTGGGAGCCCGTCTGGTACAGGAGGGGAACCGGCT
GCATTATTTAGCTGACCGGGCCAGCATCACCGGCCAGTTCAGTGACGCAGAATGCCGGAAGCTGGATGTGGCATTTCCGT
ACTTTATCCGCCAGATGGAATCCATGCTGACTACCGGTGAACTGAATCCTCGCCACACCCACTGCGTCACTCTGTACCGC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATATATCGCCATTTATCCCACTCAGCGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A241Q747