Detailed information of TA system TA065656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1314171..1314802
• Replicon: chromosome
• Accession: NZ_CP016952
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BFQ28_RS06155
• Protein ID: WP_046671263.1
• Coordinates: 1314171..1314446 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0J1MMS0
• Locus tag: BFQ28_RS06160
• Protein ID: WP_003837808.1
• Coordinates: 1314443..1314802 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS06145	1310222..1312969	+	2748	WP_032937231.1	ribosome-associated ATPase/putative transporter RbbA	-
BFQ28_RS06150	1312969..1314093	+	1125	WP_046671262.1	ABC transporter permease	-
BFQ28_RS06155	1314171..1314446	+	276	WP_046671263.1	type II toxin-antitoxin system HicA family toxin	Toxin
BFQ28_RS06160	1314443..1314802	+	360	WP_003837808.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BFQ28_RS06165	1314915..1315316	-	402	WP_003023387.1	nickel-responsive transcriptional regulator NikR	-
BFQ28_RS06170	1315366..1315944	-	579	WP_003837810.1	4'-phosphopantetheinyl transferase AcpT	-
BFQ28_RS06175	1316007..1317056	-	1050	WP_003023393.1	AI-2E family transporter	-
BFQ28_RS06180	1317190..1318407	+	1218	WP_032950436.1	MFS transporter	-
BFQ28_RS06185	1318411..1318968	-	558	WP_046671264.1	DcrB family lipoprotein	-
BFQ28_RS06190	1319041..1319706	-	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10238.90 Da
• Isoelectric Point: 10.7844

>T65656 WP_046671263.1 NZ_CP016952:1314171-1314446 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIRWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T65656 NZ_CP016952:1314171-1314446 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAGGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13198.98 Da
• Isoelectric Point: 4.5462

>AT65656 WP_003837808.1 NZ_CP016952:1314443-1314802 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT65656 NZ_CP016952:1314443-1314802 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A0J1MMS0