Detailed information of TA system TA065653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 728677..729343
• Replicon: chromosome
• Accession: NZ_CP016952
• Organism: Citrobacter freundii strain SL151

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: BFQ28_RS03455
• Protein ID: WP_046671413.1
• Coordinates: 728984..729343 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0D7LIR0
• Locus tag: BFQ28_RS03450
• Protein ID: WP_003844682.1
• Coordinates: 728677..728994 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFQ28_RS03425	724351..725175	+	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
BFQ28_RS03430	725243..726466	+	1224	WP_003031639.1	L-sorbose 1-phosphate reductase	-
BFQ28_RS03435	726468..727280	+	813	WP_044702264.1	shikimate 5-dehydrogenase	-
BFQ28_RS03440	727326..727682	-	357	WP_046671411.1	hypothetical protein	-
BFQ28_RS28380	727824..727985	+	162	WP_003841416.1	phage protein	-
BFQ28_RS03445	728389..728667	+	279	WP_046671412.1	putative addiction module antidote protein	-
BFQ28_RS03450	728677..728994	-	318	WP_003844682.1	helix-turn-helix domain-containing protein	Antitoxin
BFQ28_RS03455	728984..729343	-	360	WP_046671413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFQ28_RS03460	729557..730246	+	690	WP_046671414.1	dipeptidase PepE	-
BFQ28_RS03465	730312..731943	-	1632	WP_003031618.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13451.52 Da
• Isoelectric Point: 10.2555

>T65653 WP_046671413.1 NZ_CP016952:c729343-728984 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAIYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T65653 NZ_CP016952:c729343-728984 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGATTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11466.35 Da
• Isoelectric Point: 9.0005

>AT65653 WP_003844682.1 NZ_CP016952:c728994-728677 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT65653 NZ_CP016952:c728994-728677 [Citrobacter freundii]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGGCTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0D7LIR0