Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 402420..402936 Replicon chromosome
Accession NZ_CP016952
Organism Citrobacter freundii strain SL151

Toxin (Protein)


Gene name relE Uniprot ID A0A0J1MTE6
Locus tag BFQ28_RS01815 Protein ID WP_016149473.1
Coordinates 402420..402704 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0J1MV10
Locus tag BFQ28_RS01820 Protein ID WP_003839576.1
Coordinates 402694..402936 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFQ28_RS01800 397646..399298 + 1653 WP_016149475.1 alpha,alpha-phosphotrehalase -
BFQ28_RS01805 399707..401845 + 2139 WP_003025782.1 anaerobic ribonucleoside-triphosphate reductase -
BFQ28_RS01810 401952..402416 + 465 WP_032937736.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
BFQ28_RS01815 402420..402704 - 285 WP_016149473.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BFQ28_RS01820 402694..402936 - 243 WP_003839576.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BFQ28_RS01825 403014..404927 - 1914 WP_003844930.1 BglG family transcription antiterminator -
BFQ28_RS01830 404949..405693 - 745 Protein_357 KDGP aldolase family protein -
BFQ28_RS01835 405690..406808 - 1119 WP_046670827.1 DgaE family pyridoxal phosphate-dependent ammonia lyase -
BFQ28_RS01840 406792..407925 - 1134 WP_016149471.1 amidohydrolase/deacetylase family metallohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10878.73 Da        Isoelectric Point: 10.0482

>T65651 WP_016149473.1 NZ_CP016952:c402704-402420 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYLDANKRL

Download         Length: 285 bp

>T65651 NZ_CP016952:c402704-402420 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8961.34 Da        Isoelectric Point: 5.0651

>AT65651 WP_003839576.1 NZ_CP016952:c402936-402694 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Download         Length: 243 bp

>AT65651 NZ_CP016952:c402936-402694 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MTE6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MV10

References