Detailed information of TA system TA065646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 1906942..1907504
• Replicon: chromosome
• Accession: NZ_CP016950
• Organism: Cupriavidus taiwanensis strain SWF66294

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A375CUC1
• Locus tag: A9P79_RS20755
• Protein ID: WP_025584520.1
• Coordinates: 1906942..1907190 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A9P79_RS20760
• Protein ID: WP_115703438.1
• Coordinates: 1907184..1907504 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P79_RS20725 (A9P79_20430)	1902447..1903007	-	561	WP_115703433.1	ribosomal protein S18-alanine N-acetyltransferase	-
A9P79_RS20730 (A9P79_20435)	1903004..1903759	-	756	WP_240072221.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
A9P79_RS20735 (A9P79_20440)	1903888..1904157	+	270	WP_012353003.1	acyl-CoA-binding protein	-
A9P79_RS20740 (A9P79_20445)	1904178..1905659	-	1482	WP_115703434.1	DHA2 family efflux MFS transporter permease subunit	-
A9P79_RS20745 (A9P79_20450)	1905656..1906126	-	471	WP_172582517.1	SRPBCC family protein	-
A9P79_RS20750 (A9P79_20455)	1906368..1906775	+	408	WP_240072222.1	VOC family protein	-
A9P79_RS20755 (A9P79_20460)	1906942..1907190	+	249	WP_025584520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
A9P79_RS20760 (A9P79_20465)	1907184..1907504	+	321	WP_115703438.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
A9P79_RS20765 (A9P79_20470)	1907708..1908517	+	810	WP_240072223.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
A9P79_RS20775 (A9P79_20480)	1909030..1910586	+	1557	WP_240072224.1	serine hydrolase	-
A9P79_RS20780 (A9P79_20485)	1910654..1911541	+	888	WP_240072225.1	metal-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (7-48)

Antitoxin

• mRNA interferase PemK-like (1-103)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9037.34 Da
• Isoelectric Point: 4.5557

>T65646 WP_025584520.1 NZ_CP016950:1906942-1907190 [Cupriavidus taiwanensis]
MKATIRKMGNSQGVLIPKAILAQLGLENEVEMEIVNDTLVLRRPRQAPRQGWAEASRQIAADGDDTLVLGELPNADDAEL
KW

Nucleotide

• Length: 249 bp

>T65646 NZ_CP016950:1906942-1907190 [Cupriavidus taiwanensis]
ATGAAGGCAACGATCCGCAAGATGGGCAACTCGCAAGGTGTCCTGATTCCCAAGGCAATCCTGGCACAGCTGGGTCTGGA
GAATGAAGTGGAAATGGAAATCGTGAATGACACGCTGGTGCTGCGCCGGCCCCGGCAGGCACCGCGGCAAGGCTGGGCCG
AAGCCAGCCGGCAGATCGCCGCGGACGGCGACGACACCCTGGTGCTGGGCGAGCTGCCCAACGCCGACGACGCGGAGCTG
AAATGGTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11834.85 Da
• Isoelectric Point: 10.4426

>AT65646 WP_115703438.1 NZ_CP016950:1907184-1907504 [Cupriavidus taiwanensis]
MVARGDVWLVALDPTVGSEIEKTRPCVILSPPEMHDYLRTVMVAPMTTGSRPAPFRIPVTFQRKTGLILLDQLRTVDKSR
LVKRAGGLSDRTVSDTLRTLREVFAD

Nucleotide

• Length: 321 bp

>AT65646 NZ_CP016950:1907184-1907504 [Cupriavidus taiwanensis]
ATGGTAGCGCGCGGCGATGTCTGGCTGGTCGCGCTCGATCCCACCGTCGGCAGCGAGATCGAGAAGACGCGCCCCTGCGT
CATCCTGTCGCCGCCCGAGATGCACGACTATCTGCGCACGGTAATGGTCGCGCCGATGACCACCGGCAGCCGGCCGGCAC
CGTTTCGCATACCGGTGACGTTCCAGCGCAAGACCGGCCTGATTCTGCTGGACCAGCTGCGCACCGTCGACAAGTCGCGG
CTGGTGAAGCGCGCGGGCGGCCTAAGTGACCGGACCGTGTCAGACACCTTGCGGACCTTGCGCGAAGTGTTTGCGGACTG
A

Structures

Toxin

• AlphaFold DB: A0A375CUC1