Detailed information of TA system TA065641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1545911..1546466
• Replicon: chromosome
• Accession: NZ_CP016949
• Organism: Cupriavidus taiwanensis strain SWF66294

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: A9P79_RS06930
• Protein ID: WP_240070695.1
• Coordinates: 1546197..1546466 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: A9P79_RS06925
• Protein ID: WP_115700647.1
• Coordinates: 1545911..1546213 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P79_RS06905 (A9P79_06775)	1541356..1541622	-	267	WP_018007333.1	flagellar biosynthetic protein FliQ	-
A9P79_RS06910 (A9P79_06780)	1541624..1543774	-	2151	WP_240070693.1	type III secretion system export apparatus subunit SctV	-
A9P79_RS06915 (A9P79_06785)	1543978..1545165	+	1188	WP_240070694.1	type III secretion system cytoplasmic ring protein SctQ	-
A9P79_RS06920 (A9P79_06790)	1545209..1545868	+	660	WP_115682486.1	type III secretion system export apparatus subunit SctR	-
A9P79_RS06925 (A9P79_06795)	1545911..1546213	-	303	WP_115700647.1	BrnA antitoxin family protein	Antitoxin
A9P79_RS06930 (A9P79_06800)	1546197..1546466	-	270	WP_240070695.1	BrnT family toxin	Toxin
A9P79_RS06935 (A9P79_06805)	1546730..1547248	-	519	WP_240070696.1	N-acetyltransferase	-
A9P79_RS06940 (A9P79_06810)	1547314..1547961	-	648	WP_240070697.1	TetR/AcrR family transcriptional regulator	-
A9P79_RS06945 (A9P79_06815)	1548031..1549506	+	1476	WP_240070698.1	NAD(P)/FAD-dependent oxidoreductase	-
A9P79_RS06950 (A9P79_06820)	1549565..1550770	-	1206	WP_240070699.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1545911..1561630	15719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (33-95)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10101.34 Da
• Isoelectric Point: 6.4582

>T65641 WP_240070695.1 NZ_CP016949:c1546466-1546197 [Cupriavidus taiwanensis]
VRISFDPGKDVANTVKHGISLAMAAEIDWSDVMARPDSRSDYRELREIGFGFIGDRPYCVVFTQRGETFHVISLRKANTR
EVQDYAASY

Nucleotide

• Length: 270 bp

>T65641 NZ_CP016949:c1546466-1546197 [Cupriavidus taiwanensis]
GTGCGAATCAGCTTCGACCCCGGCAAGGATGTCGCCAACACCGTCAAGCACGGCATCTCGCTTGCCATGGCGGCCGAGAT
CGACTGGTCGGATGTAATGGCCAGGCCGGACAGCCGAAGCGACTACCGCGAGTTGCGCGAGATCGGATTCGGCTTCATCG
GGGACCGCCCCTATTGCGTGGTGTTCACGCAGCGGGGCGAGACTTTCCACGTAATCAGCCTGCGCAAGGCAAACACGCGA
GAGGTACAGGACTATGCCGCAAGCTATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11256.77 Da
• Isoelectric Point: 9.7286

>AT65641 WP_115700647.1 NZ_CP016949:c1546213-1545911 [Cupriavidus taiwanensis]
MPQAIKTRSGRTVILPTPEEDAAINRGIAADPDTYEISAEEMKQMQPLKNRGGRPRLANPKEPVTIRYDAEVLAAFRASG
DGWQTRMNDALKDWLRTHRP

Nucleotide

• Length: 303 bp

>AT65641 NZ_CP016949:c1546213-1545911 [Cupriavidus taiwanensis]
ATGCCGCAAGCTATTAAGACCCGGTCAGGCCGCACGGTCATCCTGCCGACGCCCGAAGAGGACGCGGCGATCAACCGTGG
CATTGCCGCCGACCCTGATACCTACGAGATAAGCGCAGAGGAGATGAAGCAAATGCAGCCGCTCAAGAATCGAGGCGGTC
GACCCAGGCTGGCCAACCCTAAAGAGCCAGTGACCATCCGCTATGACGCCGAAGTCCTGGCCGCCTTCCGCGCCAGTGGC
GACGGCTGGCAAACGCGGATGAATGACGCCCTGAAGGACTGGCTTCGTACACATCGTCCCTGA