Detailed information of TA system TA065617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 42914..43448
• Replicon: plasmid unnamed1
• Accession: NZ_CP016946
• Organism: Yersinia enterocolitica strain YE1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0H3NX04
• Locus tag: BFS78_RS21815
• Protein ID: WP_014609469.1
• Coordinates: 43149..43448 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P0C2N3
• Locus tag: BFS78_RS21810
• Protein ID: WP_005176524.1
• Coordinates: 42914..43156 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFS78_RS21790	38758..38976	+	219	WP_013749479.1	hypothetical protein	-
BFS78_RS21795	39037..39507	-	471	WP_010891240.1	hypothetical protein	-
BFS78_RS21800	39948..41114	+	1167	WP_010891239.1	plasmid-partitioning protein SopA	-
BFS78_RS21805	41111..42076	+	966	WP_050294053.1	ParB/RepB/Spo0J family plasmid partition protein	-
BFS78_RS23835	42456..42748	+	293	Protein_50	IS6 family transposase	-
BFS78_RS21810	42914..43156	+	243	WP_005176524.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BFS78_RS21815	43149..43448	+	300	WP_014609469.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFS78_RS21820	43589..44248	-	660	WP_072089396.1	type III secretion system effector GTPase activator YopE	-
BFS78_RS21825	44441..44833	+	393	WP_010891236.1	type III secretion system chaperone SycE/YerA	-
BFS78_RS23840	45068..46240	+	1173	Protein_55	IS3 family transposase	-
BFS78_RS21840	46444..46869	+	426	WP_010891235.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopJ/yopP / yopO/ypkA / yadA / yopE / sycE/yerA / sycH / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscR / yscQ / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV	1..73029	73029
	-	flank	IS/Tn	-	-	45615..46118	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11664.24 Da
• Isoelectric Point: 5.1053

>T65617 WP_014609469.1 NZ_CP016946:43149-43448 [Yersinia enterocolitica]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNLELGAECNWICSDMRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDAQDFPEYE

Nucleotide

• Length: 300 bp

>T65617 NZ_CP016946:43149-43448 [Yersinia enterocolitica]
GTGTATAAACTATCTGAACTGGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCTAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCAGACATGCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGCTCAGGATTTTCCAGAGTATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8856.15 Da
• Isoelectric Point: 6.7232

>AT65617 WP_005176524.1 NZ_CP016946:42914-43156 [Yersinia enterocolitica]
MARVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNAPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT65617 NZ_CP016946:42914-43156 [Yersinia enterocolitica]
ATGGCTCGCGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TACTTGCAGGAACAAATGCACCTGTGAGCCATCGTTTAATGGATGAAATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3NX04

Antitoxin

• AlphaFold DB: A1JUB1