Detailed information of TA system TA065600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 436029..437658
• Replicon: chromosome
• Accession: NZ_CP016945
• Organism: Yersinia enterocolitica strain YE1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A7U7IQ60
• Locus tag: BFS78_RS01850
• Protein ID: WP_005165219.1
• Coordinates: 436029..437357 (-)
• Length: 443 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BFS78_RS01855
• Protein ID: WP_020282668.1
• Coordinates: 437359..437658 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFS78_RS01835	431620..431973	+	354	WP_005165225.1	MmcQ/YjbR family DNA-binding protein	-
BFS78_RS01840	432032..434863	-	2832	WP_049525322.1	excinuclease ABC subunit UvrA	-
BFS78_RS01845	435392..435940	+	549	WP_049525323.1	single-stranded DNA-binding protein	-
BFS78_RS01850	436029..437357	-	1329	WP_005165219.1	type II toxin-antitoxin system HipA family toxin	Toxin
BFS78_RS01855	437359..437658	-	300	WP_020282668.1	helix-turn-helix transcriptional regulator	Antitoxin
BFS78_RS01860	437953..438624	+	672	WP_050127116.1	glutathione S-transferase	-
BFS78_RS01865	438984..440351	+	1368	WP_005165212.1	NCS2 family permease	-
BFS78_RS01870	440564..442213	+	1650	WP_020282670.1	Na+/H+ antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 443 a.a.
• Molecular weight: 49925.93 Da
• Isoelectric Point: 6.8736

>T65600 WP_005165219.1 NZ_CP016945:c437357-436029 [Yersinia enterocolitica]
MARRIQRLNIWMNGQHVGYWEKNRGEESLVYAQEWLANEQGRPLSLSLPFTANNQVYRGNIVRDYFDNLLPDSDDIRRRL
ATKYHADSINPFDLLFELGRDCVGAIQLLTDDAPPSDLFSIQQQPLTESDVARILRNSLSDNAFVRPEHDEDLRLSIAGA
QEKTALLFHDNRWCLPLGSTPTTHIFKLPLGLVGNMQADMRTSVENEWLCAKILASYNLPVASCEIGQFEDQKALIVERF
DRRLSRDKSWIVRLPQEDMCQATGTSPLKKYQMDGGPSITTIMEILSGSDNASEDRNVFFKAQIIFWLLAATDGHAKNFS
IAHLPGSRYHLTPLYDVLSTHPIIGSGRNQIAVQKSKLAMAVRGSKNYYLINQIQRRHWRKQAEFVGLGAIQADTIIDEI
IAATPRVITQVQAQLPANFPADLAEKILTGLHKQCEKISAML

Nucleotide

• Length: 1329 bp

>T65600 NZ_CP016945:c437357-436029 [Yersinia enterocolitica]
ATGGCTCGTCGCATTCAACGGCTCAATATCTGGATGAATGGTCAGCACGTTGGCTATTGGGAAAAAAATCGAGGCGAAGA
ATCGCTGGTTTATGCCCAAGAATGGTTAGCAAACGAACAAGGCCGACCACTATCATTATCATTGCCTTTCACTGCCAATA
ATCAGGTTTATAGAGGCAATATTGTCCGGGACTATTTTGATAATTTATTACCCGATAGCGATGATATCCGGCGACGTCTG
GCAACTAAATACCACGCTGACAGCATTAACCCTTTCGATCTATTGTTTGAATTAGGAAGAGATTGTGTCGGTGCAATACA
ATTGCTAACTGATGATGCTCCTCCCTCTGATCTATTCTCAATTCAACAACAACCACTAACAGAAAGCGATGTGGCTCGCA
TTCTACGAAACTCATTATCTGACAATGCATTTGTGCGCCCTGAACACGATGAGGATCTCAGATTGTCTATCGCAGGGGCA
CAAGAAAAAACAGCTCTTTTGTTTCACGACAACCGATGGTGCCTCCCATTGGGCAGCACTCCCACCACCCATATCTTCAA
ACTTCCGTTGGGCTTAGTCGGCAATATGCAAGCTGATATGAGGACCTCGGTTGAAAATGAATGGCTGTGTGCAAAAATTC
TGGCAAGTTATAACTTACCGGTCGCCAGTTGTGAAATTGGCCAGTTTGAAGACCAAAAAGCATTGATTGTTGAACGTTTT
GACCGACGTTTATCGCGTGACAAGAGTTGGATCGTCAGATTACCGCAAGAAGATATGTGCCAGGCCACTGGGACTTCTCC
CTTAAAAAAATATCAAATGGATGGTGGCCCCAGTATTACCACCATAATGGAGATTTTGAGTGGTTCGGATAATGCCAGTG
AAGACAGAAACGTCTTCTTTAAAGCACAGATTATTTTCTGGTTATTGGCCGCAACTGATGGACATGCAAAAAACTTTAGC
ATTGCTCATCTCCCCGGGAGTCGTTATCACCTCACGCCACTTTATGACGTGTTATCAACCCATCCGATTATCGGCTCGGG
TCGTAATCAGATTGCGGTTCAAAAATCCAAGTTAGCGATGGCGGTAAGAGGGAGTAAAAATTACTATTTGATTAACCAGA
TCCAACGCCGTCATTGGCGAAAACAGGCAGAATTTGTTGGACTAGGAGCGATTCAAGCTGACACTATTATCGATGAAATA
ATTGCCGCAACCCCACGGGTTATCACACAAGTACAAGCTCAACTTCCGGCTAATTTTCCTGCTGACTTAGCAGAGAAAAT
CCTTACTGGCCTTCATAAACAATGTGAAAAAATCTCGGCAATGCTTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11155.81 Da
• Isoelectric Point: 8.5374

>AT65600 WP_020282668.1 NZ_CP016945:c437658-437359 [Yersinia enterocolitica]
MDYPIKILSQLRPTLIGFRKNKGLTQASLAQLLGITQQSYAKLEADPTSASVERLFKVLQLLDVELILTEKKNASAHPNK
THQPEQSQTIDLPSKQEDW

Nucleotide

• Length: 300 bp

>AT65600 NZ_CP016945:c437658-437359 [Yersinia enterocolitica]
ATGGACTATCCCATTAAAATTTTGAGTCAATTACGCCCGACGTTGATTGGTTTTCGAAAAAATAAAGGGCTGACTCAGGC
CAGCCTGGCACAACTGTTAGGTATCACCCAGCAGTCCTATGCAAAGCTTGAAGCTGATCCCACCTCGGCCAGTGTTGAAC
GGCTTTTTAAAGTATTACAACTTTTAGACGTAGAACTGATTCTCACTGAGAAAAAAAACGCCAGCGCGCATCCCAATAAA
ACCCACCAGCCAGAACAAAGCCAGACGATAGATTTACCGTCAAAACAAGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U7IQ60