Detailed information of TA system TA065599

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 106692..107276
• Replicon: chromosome
• Accession: NZ_CP016945
• Organism: Yersinia enterocolitica strain YE1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7U7ISC5
• Locus tag: BFS78_RS00450
• Protein ID: WP_005165296.1
• Coordinates: 106692..107060 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BFS78_RS22505
• Protein ID: WP_129019424.1
• Coordinates: 107124..107276 (-)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFS78_RS00430	102000..102806	+	807	Protein_78	ROK family protein	-
BFS78_RS00435	102891..103853	+	963	WP_005165289.1	LacI family transcriptional regulator	-
BFS78_RS00440	103887..104360	-	474	WP_049528679.1	GNAT family N-acetyltransferase	-
BFS78_RS00445	105609..106463	-	855	WP_049528676.1	DUF4942 domain-containing protein	-
BFS78_RS00450	106692..107060	-	369	WP_005165296.1	TA system toxin CbtA family protein	Toxin
BFS78_RS22505	107124..107276	-	153	WP_129019424.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BFS78_RS00460	107345..108548	+	1204	Protein_84	IS256 family transposase	-
BFS78_RS00465	108723..110186	+	1464	WP_005166182.1	alpha/beta hydrolase	-
BFS78_RS00470	110419..111825	-	1407	WP_069008916.1	filamentous hemagglutinin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	87047..114994	27947
	-	flank	IS/Tn	-	-	100917..101948	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (1-45)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.55 Da
• Isoelectric Point: 6.4731

>T65599 WP_005165296.1 NZ_CP016945:c107060-106692 [Yersinia enterocolitica]
MQTLPVTSKRAAYACLSPVIVWQSMLTYLLEQHYGLVLSDTEFSDDAVIHEHIDAGISLADALNFTVEKFDLARTDHRGF
SCQEQSPFITSIDILRARRATGLMTRMGYQTITSVIRGGKQA

Nucleotide

• Length: 369 bp

>T65599 NZ_CP016945:c107060-106692 [Yersinia enterocolitica]
ATGCAGACCTTACCTGTCACCTCGAAACGGGCGGCCTACGCTTGCCTATCACCCGTTATTGTCTGGCAATCCATGCTCAC
GTATCTGCTAGAGCAGCATTACGGCCTGGTGCTTAGCGACACCGAGTTCAGTGATGACGCCGTTATCCACGAACATATTG
ATGCCGGTATCTCTCTGGCTGATGCGCTGAATTTCACGGTGGAGAAGTTTGACTTGGCTCGCACCGATCACCGAGGTTTC
TCCTGCCAGGAACAATCTCCCTTTATCACGTCTATCGATATTCTCCGCGCTCGTCGAGCCACCGGTCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGGAAAGCAGGCATGA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5593.44 Da
• Isoelectric Point: 4.5803

>AT65599 WP_129019424.1 NZ_CP016945:c107276-107124 [Yersinia enterocolitica]
MELMLLSGELNPRYQHCVTLYRNGLICEADSLGSQGYVYLAIYPTPQSIA

Nucleotide

• Length: 153 bp

>AT65599 NZ_CP016945:c107276-107124 [Yersinia enterocolitica]
CTGGAACTGATGCTGCTTTCTGGTGAGCTCAATCCCCGTTATCAGCACTGCGTCACGTTGTATCGCAATGGCTTGATCTG
CGAAGCCGATAGCCTGGGATCACAGGGTTATGTGTATCTCGCCATTTATCCTACTCCCCAATCAATTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U7ISC5