Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1248050..1248668 Replicon chromosome
Accession NZ_CP016940
Organism Yersinia enterocolitica strain YE5

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag BED32_RS06180 Protein ID WP_002208622.1
Coordinates 1248050..1248253 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7U7FKE5
Locus tag BED32_RS06185 Protein ID WP_005163463.1
Coordinates 1248300..1248668 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BED32_RS06150 1243421..1243759 + 339 WP_002208627.1 P-II family nitrogen regulator -
BED32_RS06155 1243799..1245088 + 1290 WP_005167660.1 ammonium transporter AmtB -
BED32_RS06160 1245202..1246065 - 864 WP_005163474.1 acyl-CoA thioesterase II -
BED32_RS06165 1246326..1246856 + 531 WP_005163468.1 YbaY family lipoprotein -
BED32_RS06170 1246908..1247213 - 306 WP_005163466.1 MGMT family protein -
BED32_RS06180 1248050..1248253 - 204 WP_002208622.1 expression modulating protein YmoA Toxin
BED32_RS06185 1248300..1248668 - 369 WP_005163463.1 Hha toxicity modulator TomB Antitoxin
BED32_RS06190 1248826..1249179 - 354 WP_005163460.1 hypothetical protein -
BED32_RS06195 1249945..1250655 + 711 WP_013649453.1 ABC transporter ATP-binding protein -
BED32_RS06200 1250669..1251526 + 858 WP_020283451.1 metal ABC transporter permease -
BED32_RS06205 1251589..1252467 + 879 WP_049525734.1 metal ABC transporter substrate-binding protein -
BED32_RS06210 1252595..1252738 - 144 WP_002208618.1 type B 50S ribosomal protein L36 -
BED32_RS06215 1252754..1253014 - 261 WP_005163438.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T65565 WP_002208622.1 NZ_CP016940:c1248253-1248050 [Yersinia enterocolitica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T65565 NZ_CP016940:c1248253-1248050 [Yersinia enterocolitica]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATTGATACATTGGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGACGATGAGCTGGAATTGTTTTACTCAGCAGCAGACCACCGCTTAGCTGAACTCACAATGAATAAAC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14192.90 Da        Isoelectric Point: 4.3377

>AT65565 WP_005163463.1 NZ_CP016940:c1248668-1248300 [Yersinia enterocolitica]
MDEYSPKRHDVAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDDGDLSELVEEYL
DDTYTLFSSYGINDPELQRWQKTKERLFRLFSGEYICTLMKT

Download         Length: 369 bp

>AT65565 NZ_CP016940:c1248668-1248300 [Yersinia enterocolitica]
ATGGATGAGTACTCACCTAAGCGGCATGACGTTGCCCAATTGAAGTTCCTGTGCGAGAGTTTGTATGATGAAGGCATTGC
TACCCTTGGTGATAGCCATCATGGCTGGGTAAATGATCCAACTTCAGCCGTAAACCTGCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGACGGCGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTACGGAATCAATGATCCGGAATTGCAGCGCTGGCAAAAAACTAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
PDB 2MN2
AlphaFold DB A0A7U7FKE5

References