Detailed information of TA system TA065563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 436387..438016
• Replicon: chromosome
• Accession: NZ_CP016940
• Organism: Yersinia enterocolitica strain YE5

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A7U7IQ60
• Locus tag: BED32_RS02305
• Protein ID: WP_005165219.1
• Coordinates: 436387..437715 (-)
• Length: 443 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: BED32_RS02310
• Protein ID: WP_020282668.1
• Coordinates: 437717..438016 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED32_RS02290	431978..432331	+	354	WP_005165225.1	MmcQ/YjbR family DNA-binding protein	-
BED32_RS02295	432390..435221	-	2832	WP_049525322.1	excinuclease ABC subunit UvrA	-
BED32_RS02300	435750..436298	+	549	WP_049525323.1	single-stranded DNA-binding protein	-
BED32_RS02305	436387..437715	-	1329	WP_005165219.1	type II toxin-antitoxin system HipA family toxin	Toxin
BED32_RS02310	437717..438016	-	300	WP_020282668.1	helix-turn-helix transcriptional regulator	Antitoxin
BED32_RS02315	438311..438982	+	672	WP_050127116.1	glutathione S-transferase	-
BED32_RS02320	439342..440709	+	1368	WP_005165212.1	NCS2 family permease	-
BED32_RS02325	440922..442571	+	1650	WP_020282670.1	Na+/H+ antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (8-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 443 a.a.
• Molecular weight: 49925.93 Da
• Isoelectric Point: 6.8736

>T65563 WP_005165219.1 NZ_CP016940:c437715-436387 [Yersinia enterocolitica]
MARRIQRLNIWMNGQHVGYWEKNRGEESLVYAQEWLANEQGRPLSLSLPFTANNQVYRGNIVRDYFDNLLPDSDDIRRRL
ATKYHADSINPFDLLFELGRDCVGAIQLLTDDAPPSDLFSIQQQPLTESDVARILRNSLSDNAFVRPEHDEDLRLSIAGA
QEKTALLFHDNRWCLPLGSTPTTHIFKLPLGLVGNMQADMRTSVENEWLCAKILASYNLPVASCEIGQFEDQKALIVERF
DRRLSRDKSWIVRLPQEDMCQATGTSPLKKYQMDGGPSITTIMEILSGSDNASEDRNVFFKAQIIFWLLAATDGHAKNFS
IAHLPGSRYHLTPLYDVLSTHPIIGSGRNQIAVQKSKLAMAVRGSKNYYLINQIQRRHWRKQAEFVGLGAIQADTIIDEI
IAATPRVITQVQAQLPANFPADLAEKILTGLHKQCEKISAML

Nucleotide

• Length: 1329 bp

>T65563 NZ_CP016940:c437715-436387 [Yersinia enterocolitica]
ATGGCTCGTCGCATTCAACGGCTCAATATCTGGATGAATGGTCAGCACGTTGGCTATTGGGAAAAAAATCGAGGCGAAGA
ATCGCTGGTTTATGCCCAAGAATGGTTAGCAAACGAACAAGGCCGACCACTATCATTATCATTGCCTTTCACTGCCAATA
ATCAGGTTTATAGAGGCAATATTGTCCGGGACTATTTTGATAATTTATTACCCGATAGCGATGATATCCGGCGACGTCTG
GCAACTAAATACCACGCTGACAGCATTAACCCTTTCGATCTATTGTTTGAATTAGGAAGAGATTGTGTCGGTGCAATACA
ATTGCTAACTGATGATGCTCCTCCCTCTGATCTATTCTCAATTCAACAACAACCACTAACAGAAAGCGATGTGGCTCGCA
TTCTACGAAACTCATTATCTGACAATGCATTTGTGCGCCCTGAACACGATGAGGATCTCAGATTGTCTATCGCAGGGGCA
CAAGAAAAAACAGCTCTTTTGTTTCACGACAACCGATGGTGCCTCCCATTGGGCAGCACTCCCACCACCCATATCTTCAA
ACTTCCGTTGGGCTTAGTCGGCAATATGCAAGCTGATATGAGGACCTCGGTTGAAAATGAATGGCTGTGTGCAAAAATTC
TGGCAAGTTATAACTTACCGGTCGCCAGTTGTGAAATTGGCCAGTTTGAAGACCAAAAAGCATTGATTGTTGAACGTTTT
GACCGACGTTTATCGCGTGACAAGAGTTGGATCGTCAGATTACCGCAAGAAGATATGTGCCAGGCCACTGGGACTTCTCC
CTTAAAAAAATATCAAATGGATGGTGGCCCCAGTATTACCACCATAATGGAGATTTTGAGTGGTTCGGATAATGCCAGTG
AAGACAGAAACGTCTTCTTTAAAGCACAGATTATTTTCTGGTTATTGGCCGCAACTGATGGACATGCAAAAAACTTTAGC
ATTGCTCATCTCCCCGGGAGTCGTTATCACCTCACGCCACTTTATGACGTGTTATCAACCCATCCGATTATCGGCTCGGG
TCGTAATCAGATTGCGGTTCAAAAATCCAAGTTAGCGATGGCGGTAAGAGGGAGTAAAAATTACTATTTGATTAACCAGA
TCCAACGCCGTCATTGGCGAAAACAGGCAGAATTTGTTGGACTAGGAGCGATTCAAGCTGACACTATTATCGATGAAATA
ATTGCCGCAACCCCACGGGTTATCACACAAGTACAAGCTCAACTTCCGGCTAATTTTCCTGCTGACTTAGCAGAGAAAAT
CCTTACTGGCCTTCATAAACAATGTGAAAAAATCTCGGCAATGCTTTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11155.81 Da
• Isoelectric Point: 8.5374

>AT65563 WP_020282668.1 NZ_CP016940:c438016-437717 [Yersinia enterocolitica]
MDYPIKILSQLRPTLIGFRKNKGLTQASLAQLLGITQQSYAKLEADPTSASVERLFKVLQLLDVELILTEKKNASAHPNK
THQPEQSQTIDLPSKQEDW

Nucleotide

• Length: 300 bp

>AT65563 NZ_CP016940:c438016-437717 [Yersinia enterocolitica]
ATGGACTATCCCATTAAAATTTTGAGTCAATTACGCCCGACGTTGATTGGTTTTCGAAAAAATAAAGGGCTGACTCAGGC
CAGCCTGGCACAACTGTTAGGTATCACCCAGCAGTCCTATGCAAAGCTTGAAGCTGATCCCACCTCGGCCAGTGTTGAAC
GGCTTTTTAAAGTATTACAACTTTTAGACGTAGAACTGATTCTCACTGAGAAAAAAAACGCCAGCGCGCATCCCAATAAA
ACCCACCAGCCAGAACAAAGCCAGACGATAGATTTACCGTCAAAACAAGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U7IQ60