Detailed information of TA system TA065561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 42914..43448
• Replicon: plasmid unnamed1
• Accession: NZ_CP016939
• Organism: Yersinia enterocolitica strain YE5

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0H3NX04
• Locus tag: BED32_RS00265
• Protein ID: WP_014609469.1
• Coordinates: 43149..43448 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P0C2N3
• Locus tag: BED32_RS00260
• Protein ID: WP_005176524.1
• Coordinates: 42914..43156 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED32_RS00235	38757..38975	+	219	WP_013749479.1	hypothetical protein	-
BED32_RS00240	39036..39506	-	471	WP_010891240.1	hypothetical protein	-
BED32_RS00250	39948..41114	+	1167	WP_010891239.1	plasmid-partitioning protein SopA	-
BED32_RS00255	41111..42076	+	966	WP_050294053.1	ParB/RepB/Spo0J family plasmid partition protein	-
BED32_RS23290	42456..42748	+	293	Protein_50	IS6 family transposase	-
BED32_RS00260	42914..43156	+	243	WP_005176524.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BED32_RS00265	43149..43448	+	300	WP_014609469.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BED32_RS00270	43589..44248	-	660	WP_072089396.1	type III secretion system effector GTPase activator YopE	-
BED32_RS00275	44441..44833	+	393	WP_010891236.1	type III secretion system chaperone SycE/YerA	-
BED32_RS23295	45068..46240	+	1173	Protein_55	IS3 family transposase	-
BED32_RS00290	46444..46869	+	426	WP_010891235.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopJ/yopP / yopO/ypkA / yadA / yopE / sycE/yerA / sycH / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscO / yscN / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV	1..73034	73034
	-	flank	IS/Tn	-	-	45615..46118	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11664.24 Da
• Isoelectric Point: 5.1053

>T65561 WP_014609469.1 NZ_CP016939:43149-43448 [Yersinia enterocolitica]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNLELGAECNWICSDMRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDAQDFPEYE

Nucleotide

• Length: 300 bp

>T65561 NZ_CP016939:43149-43448 [Yersinia enterocolitica]
GTGTATAAACTATCTGAACTGGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCTAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCAGACATGCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGCTCAGGATTTTCCAGAGTATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8856.15 Da
• Isoelectric Point: 6.7232

>AT65561 WP_005176524.1 NZ_CP016939:42914-43156 [Yersinia enterocolitica]
MARVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNAPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT65561 NZ_CP016939:42914-43156 [Yersinia enterocolitica]
ATGGCTCGCGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TACTTGCAGGAACAAATGCACCTGTGAGCCATCGTTTAATGGATGAAATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3NX04

Antitoxin

• AlphaFold DB: A1JUB1