Detailed information of TA system TA065521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 43155..43689
• Replicon: plasmid unnamed2
• Accession: NZ_CP016933
• Organism: Yersinia enterocolitica strain YE165

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0H3NX04
• Locus tag: BB936_RS22540
• Protein ID: WP_014609469.1
• Coordinates: 43390..43689 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P0C2N3
• Locus tag: BB936_RS22535
• Protein ID: WP_005176524.1
• Coordinates: 43155..43397 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB936_RS22510	38998..39216	+	219	WP_013749479.1	hypothetical protein	-
BB936_RS22515	39277..39747	-	471	WP_010891240.1	hypothetical protein	-
BB936_RS22525	40189..41355	+	1167	WP_010891239.1	plasmid-partitioning protein SopA	-
BB936_RS22530	41352..42317	+	966	WP_050294053.1	ParB/RepB/Spo0J family plasmid partition protein	-
BB936_RS24225	42697..42989	+	293	Protein_50	IS6 family transposase	-
BB936_RS22535	43155..43397	+	243	WP_005176524.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BB936_RS22540	43390..43689	+	300	WP_014609469.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BB936_RS22545	43830..44489	-	660	WP_072089396.1	type III secretion system effector GTPase activator YopE	-
BB936_RS22550	44682..45074	+	393	WP_010891236.1	type III secretion system chaperone SycE/YerA	-
BB936_RS24230	45309..46481	+	1173	Protein_55	IS3 family transposase	-
BB936_RS22565	46685..47110	+	426	WP_010891235.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopD / yopM / sycT / yopT / yopK/yopQ / yopJ/yopP / yopO/ypkA / yopO/ypkA / yadA / yopE / sycE/yerA / sycH / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscQ / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB	1..74497	74497
	-	flank	IS/Tn	-	-	45856..46359	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11664.24 Da
• Isoelectric Point: 5.1053

>T65521 WP_014609469.1 NZ_CP016933:43390-43689 [Yersinia enterocolitica]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNLELGAECNWICSDMRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDAQDFPEYE

Nucleotide

• Length: 300 bp

>T65521 NZ_CP016933:43390-43689 [Yersinia enterocolitica]
GTGTATAAACTATCTGAACTGGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCTAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCAGACATGCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGCTCAGGATTTTCCAGAGTATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8856.15 Da
• Isoelectric Point: 6.7232

>AT65521 WP_005176524.1 NZ_CP016933:43155-43397 [Yersinia enterocolitica]
MARVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNAPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT65521 NZ_CP016933:43155-43397 [Yersinia enterocolitica]
ATGGCTCGCGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TACTTGCAGGAACAAATGCACCTGTGAGCCATCGTTTAATGGATGAAATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3NX04

Antitoxin

• AlphaFold DB: A1JUB1