Detailed information of TA system TA065504

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 268039..268623
• Replicon: chromosome
• Accession: NZ_CP016931
• Organism: Yersinia enterocolitica strain YE165

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7U7ISC5
• Locus tag: BB936_RS01125
• Protein ID: WP_005165296.1
• Coordinates: 268039..268407 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BB936_RS22810
• Protein ID: WP_129019424.1
• Coordinates: 268471..268623 (-)
• Length: 51 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB936_RS01105	263347..264153	+	807	Protein_216	ROK family protein	-
BB936_RS01110	264238..265200	+	963	WP_005165289.1	LacI family transcriptional regulator	-
BB936_RS01115	265234..265707	-	474	WP_049528679.1	GNAT family N-acetyltransferase	-
BB936_RS01120	266956..267810	-	855	WP_049528676.1	DUF4942 domain-containing protein	-
BB936_RS01125	268039..268407	-	369	WP_005165296.1	TA system toxin CbtA family protein	Toxin
BB936_RS22810	268471..268623	-	153	WP_129019424.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BB936_RS01135	268692..269895	+	1204	Protein_222	IS256 family transposase	-
BB936_RS01140	270070..271533	+	1464	WP_005166182.1	alpha/beta hydrolase	-
BB936_RS01145	271766..273172	-	1407	WP_057618982.1	filamentous hemagglutinin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	248419..276341	27922
	-	flank	IS/Tn	-	-	262264..263295	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (1-45)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13609.55 Da
• Isoelectric Point: 6.4731

>T65504 WP_005165296.1 NZ_CP016931:c268407-268039 [Yersinia enterocolitica]
MQTLPVTSKRAAYACLSPVIVWQSMLTYLLEQHYGLVLSDTEFSDDAVIHEHIDAGISLADALNFTVEKFDLARTDHRGF
SCQEQSPFITSIDILRARRATGLMTRMGYQTITSVIRGGKQA

Nucleotide

• Length: 369 bp

>T65504 NZ_CP016931:c268407-268039 [Yersinia enterocolitica]
ATGCAGACCTTACCTGTCACCTCGAAACGGGCGGCCTACGCTTGCCTATCACCCGTTATTGTCTGGCAATCCATGCTCAC
GTATCTGCTAGAGCAGCATTACGGCCTGGTGCTTAGCGACACCGAGTTCAGTGATGACGCCGTTATCCACGAACATATTG
ATGCCGGTATCTCTCTGGCTGATGCGCTGAATTTCACGGTGGAGAAGTTTGACTTGGCTCGCACCGATCACCGAGGTTTC
TCCTGCCAGGAACAATCTCCCTTTATCACGTCTATCGATATTCTCCGCGCTCGTCGAGCCACCGGTCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGGAAAGCAGGCATGA

Antitoxin

Protein

• Length: 51 a.a.
• Molecular weight: 5593.44 Da
• Isoelectric Point: 4.5803

>AT65504 WP_129019424.1 NZ_CP016931:c268623-268471 [Yersinia enterocolitica]
MELMLLSGELNPRYQHCVTLYRNGLICEADSLGSQGYVYLAIYPTPQSIA

Nucleotide

• Length: 153 bp

>AT65504 NZ_CP016931:c268623-268471 [Yersinia enterocolitica]
CTGGAACTGATGCTGCTTTCTGGTGAGCTCAATCCCCGTTATCAGCACTGCGTCACGTTGTATCGCAATGGCTTGATCTG
CGAAGCCGATAGCCTGGGATCACAGGGTTATGTGTATCTCGCCATTTATCCTACTCCCCAATCAATTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U7ISC5