Detailed information of TA system TA065502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4703352..4703905
• Replicon: chromosome
• Accession: NZ_CP016928
• Organism: Dickeya solani RNS 08.23.3.1.A

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: D083_RS20610
• Protein ID: WP_022633101.1
• Coordinates: 4703591..4703905 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2K8W593
• Locus tag: D083_RS20605
• Protein ID: WP_022633100.1
• Coordinates: 4703352..4703588 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D083_RS20590	4698928..4700577	+	1650	WP_022633097.1	hypothetical protein	-
D083_RS20595	4700938..4701339	-	402	WP_023637748.1	hypothetical protein	-
D083_RS21855	4701403..4701696	-	294	WP_146053334.1	hypothetical protein	-
D083_RS20600	4702087..4703031	+	945	WP_022633099.1	DUF1738 domain-containing protein	-
D083_RS20605	4703352..4703588	+	237	WP_022633100.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
D083_RS20610	4703591..4703905	+	315	WP_022633101.1	CcdB family protein	Toxin
D083_RS20615	4703902..4704159	-	258	WP_022633102.1	hypothetical protein	-
D083_RS20620	4704335..4705642	-	1308	WP_022633103.1	type II toxin-antitoxin system HipA family toxin	-
D083_RS20625	4705642..4706049	-	408	WP_071598628.1	helix-turn-helix transcriptional regulator	-
D083_RS20635	4706432..4707178	-	747	WP_022633104.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hcp	4694369..4742747	48378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11459.38 Da
• Isoelectric Point: 7.9859

>T65502 WP_022633101.1 NZ_CP016928:4703591-4703905 [Dickeya solani RNS 08.23.3.1.A]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGI

Nucleotide

• Length: 315 bp

>T65502 NZ_CP016928:4703591-4703905 [Dickeya solani RNS 08.23.3.1.A]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCTGTTTACCCGCTGTTGCTCGATGTCACAAGCGATATTATTGG
GCAGTTGAATCGCCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTATCCGGCAGGCCGCCGACCAGATCGTCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCACTGGGG
GCGGTGTTTTGTGATGCGTCTCAGTATCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATCGACGGCATTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9020.06 Da
• Isoelectric Point: 6.2206

>AT65502 WP_022633100.1 NZ_CP016928:4703352-4703588 [Dickeya solani RNS 08.23.3.1.A]
MTAKQRNTQSVTMTVERALLVRAREAGINLSAILTTALDAELRHHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT65502 NZ_CP016928:4703352-4703588 [Dickeya solani RNS 08.23.3.1.A]
ATGACCGCGAAACAACGCAACACGCAAAGCGTGACCATGACGGTGGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
CATTAACCTGAGTGCCATCCTGACAACCGCCCTGGATGCAGAGCTTCGCCACCATGAAGCAAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAACCGTTTTCATGATGAAAATGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W593