Detailed information of TA system TA065496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3413656..3414440
• Replicon: chromosome
• Accession: NZ_CP016928
• Organism: Dickeya solani RNS 08.23.3.1.A

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: D083_RS14970
• Protein ID: WP_022632047.1
• Coordinates: 3413946..3414440 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2K8W1V4
• Locus tag: D083_RS14965
• Protein ID: WP_022632046.1
• Coordinates: 3413656..3413949 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D083_RS14945	3409482..3409934	+	453	WP_022632042.1	hypothetical protein	-
D083_RS14950	3409977..3410273	+	297	WP_022632043.1	hypothetical protein	-
D083_RS14955	3410301..3411008	+	708	WP_022632044.1	SDR family oxidoreductase	-
D083_RS14960	3412000..3413251	-	1252	Protein_2881	tyrosine-type recombinase/integrase	-
D083_RS14965	3413656..3413949	+	294	WP_022632046.1	DUF1778 domain-containing protein	Antitoxin
D083_RS14970	3413946..3414440	+	495	WP_022632047.1	GNAT family N-acetyltransferase	Toxin
D083_RS14975	3414492..3414889	+	398	Protein_2884	DUF3577 domain-containing protein	-
D083_RS14980	3415005..3416276	+	1272	WP_022632048.1	hypothetical protein	-
D083_RS14985	3416280..3416846	+	567	WP_024103901.1	hypothetical protein	-
D083_RS14990	3416834..3418774	+	1941	WP_022632050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3413413..3423732	10319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17639.41 Da
• Isoelectric Point: 6.8524

>T65496 WP_022632047.1 NZ_CP016928:3413946-3414440 [Dickeya solani RNS 08.23.3.1.A]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSRVLAYYSLASSAVATTAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T65496 NZ_CP016928:3413946-3414440 [Dickeya solani RNS 08.23.3.1.A]
ATGATCTCTGCTCCTGAGCCACTCCACGCCGAACATGTACTATCTTCATTCTGTTGCGGTGTGGAGTCTATGGACAACTG
GCTGAAACAACGGGCGATGAAAAATCAGGTCACTGGCGCATCCCGAACTTTCGTCAGTTGCGATGATTCAAGAGTGTTGG
CGTATTACTCATTGGCTTCCAGCGCCGTGGCAACAACTGCCGCCCCCGGACGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTGGTAGTGCTGGGACGTCTGGCGGTTGATACGTCATTGCATGGTCAGGGCGTTGGCCGGGCGCTGGTGCGTGATGC
CGGGCTACGGGTGATTCAGGTAGCGGATACTATCGGCATTCGCGGGATGTTGGTTCATGCGCTGTCTGATGAAGCGCGTG
ATTTTTACCTACGAGTGGGGTTTGAACCTTCACCGATTGACCCGATGATTTTGATGGTAACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.53 Da
• Isoelectric Point: 9.8078

>AT65496 WP_022632046.1 NZ_CP016928:3413656-3413949 [Dickeya solani RNS 08.23.3.1.A]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT65496 NZ_CP016928:3413656-3413949 [Dickeya solani RNS 08.23.3.1.A]
ATGCCAGTGACGAACAGCGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCCGAACGCGATCTGATCGA
TCGTGCAGCGAAGGCCAGAGGAAAAAATCGTACTGATTTTGTTCTTGAGGCTGCTCGCGCCGCCGCAGAGGAAGCGTTGA
TCGATCAGCGTATCATCATGGCAGATCCAGATGCCTATCAGGAATTCCTTGCTCGCTTGGATCAGGCTCCTGAGCCAAAT
GCCGCGCTGCGTAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V4