Detailed information of TA system TA065466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2415..2940
• Replicon: plasmid pCTXM15_DHQP1400954
• Accession: NZ_CP016925
• Organism: Klebsiella pneumoniae isolate 23

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: A8C23_RS00610
• Protein ID: WP_004197633.1
• Coordinates: 2415..2720 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: A8C23_RS00615
• Protein ID: WP_004197642.1
• Coordinates: 2722..2940 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8C23_RS00595	482..1498	-	1017	WP_004197639.1	hypothetical protein	-
A8C23_RS00600	1495..1818	-	324	WP_004197641.1	hypothetical protein	-
A8C23_RS00605	1845..2246	-	402	WP_072199347.1	hypothetical protein	-
A8C23_RS00610	2415..2720	-	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
A8C23_RS00615	2722..2940	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
A8C23_RS00620	3536..3784	+	249	WP_019725036.1	hypothetical protein	-
A8C23_RS00625	3781..4353	+	573	WP_015065504.1	hypothetical protein	-
A8C23_RS00630	4384..4878	+	495	WP_009310053.1	hypothetical protein	-
A8C23_RS00635	4922..5290	+	369	WP_015065502.1	hypothetical protein	-
A8C23_RS00640	5324..5527	+	204	WP_019725040.1	hemolysin expression modulator Hha	-
A8C23_RS00645	5541..5771	+	231	WP_019725034.1	hypothetical protein	-
A8C23_RS29195	5815..6009	-	195	WP_019725033.1	hypothetical protein	-
A8C23_RS00655	6443..6766	-	324	WP_019725650.1	hypothetical protein	-
A8C23_RS00660	6839..7834	-	996	WP_019725651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A)	-	1..63291	63291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T65466 WP_004197633.1 NZ_CP016925:c2720-2415 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T65466 NZ_CP016925:c2720-2415 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT65466 WP_004197642.1 NZ_CP016925:c2940-2722 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT65466 NZ_CP016925:c2940-2722 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5