Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1999528..2000153 Replicon chromosome
Accession NZ_CP016923
Organism Klebsiella pneumoniae isolate 11

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag A8C11_RS12030 Protein ID WP_002882817.1
Coordinates 1999770..2000153 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag A8C11_RS12025 Protein ID WP_004150355.1
Coordinates 1999528..1999770 (+) Length 81 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8C11_RS12000 1995519..1996118 + 600 WP_004151865.1 glucose-1-phosphatase -
A8C11_RS12005 1996112..1996972 + 861 WP_004146232.1 virulence factor BrkB family protein -
A8C11_RS12010 1996969..1997406 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
A8C11_RS12015 1997451..1998392 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
A8C11_RS12020 1998406..1999323 - 918 WP_004181612.1 alpha/beta hydrolase -
A8C11_RS12025 1999528..1999770 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
A8C11_RS12030 1999770..2000153 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
A8C11_RS12035 2000327..2001256 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
A8C11_RS12040 2001253..2001888 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
A8C11_RS12045 2001885..2002787 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-112)

Antitoxin

(16-47)


Sequences


Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T65451 WP_002882817.1 NZ_CP016923:1999770-2000153 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T65451 NZ_CP016923:1999770-2000153 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT65451 WP_004150355.1 NZ_CP016923:1999528-1999770 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT65451 NZ_CP016923:1999528-1999770 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References