Detailed information of TA system TA065449

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1093336..1093993
• Replicon: chromosome
• Accession: NZ_CP016923
• Organism: Klebsiella pneumoniae isolate 11

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: A8C11_RS07560
• Protein ID: WP_004181233.1
• Coordinates: 1093336..1093746 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: A8C11_RS07565
• Protein ID: WP_002916312.1
• Coordinates: 1093727..1093993 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8C11_RS07540	1089336..1091069	-	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-
A8C11_RS07545	1091075..1091788	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
A8C11_RS07550	1091811..1092707	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
A8C11_RS07555	1092808..1093329	+	522	WP_004144730.1	flavodoxin FldB	-
A8C11_RS07560	1093336..1093746	-	411	WP_004181233.1	protein YgfX	Toxin
A8C11_RS07565	1093727..1093993	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
A8C11_RS07570	1094239..1095222	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
A8C11_RS07575	1095373..1096032	-	660	WP_002916317.1	hemolysin III family protein	-
A8C11_RS07580	1096196..1096507	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
A8C11_RS07585	1096557..1097285	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
A8C11_RS07590	1097404..1098837	+	1434	WP_004181234.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T65449 WP_004181233.1 NZ_CP016923:c1093746-1093336 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T65449 NZ_CP016923:c1093746-1093336 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGAAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT65449 WP_002916312.1 NZ_CP016923:c1093993-1093727 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT65449 NZ_CP016923:c1093993-1093727 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41