Detailed information of TA system TA065447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 234421..235331
• Replicon: plasmid pIncHI1B_DHQP1300920
• Accession: NZ_CP016921
• Organism: Klebsiella pneumoniae isolate 11

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: A8C11_RS01600
• Protein ID: WP_004181896.1
• Coordinates: 234861..235331 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: A8C11_RS01595
• Protein ID: WP_004181895.1
• Coordinates: 234421..234864 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8C11_RS01580	231054..232541	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
A8C11_RS01585	232627..233049	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
A8C11_RS01590	233049..234320	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
A8C11_RS01595	234421..234864	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
A8C11_RS01600	234861..235331	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
A8C11_RS01605	235441..235701	-	261	WP_004026352.1	hypothetical protein	-
A8C11_RS01610	236424..236582	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
A8C11_RS32425	237185..238159	+	975	WP_043907013.1	hypothetical protein	-
A8C11_RS01620	238357..238734	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1	-	1..283369	283369

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T65447 WP_004181896.1 NZ_CP016921:234861-235331 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T65447 NZ_CP016921:234861-235331 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT65447 WP_004181895.1 NZ_CP016921:234421-234864 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT65447 NZ_CP016921:234421-234864 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9