Detailed information of TA system TA065445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 64662..65305
• Replicon: plasmid pIncR_DHQP1300920
• Accession: NZ_CP016919
• Organism: Klebsiella pneumoniae isolate 11

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: A8C11_RS00385
• Protein ID: WP_001044770.1
• Coordinates: 64662..65078 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: A8C11_RS00390
• Protein ID: WP_001261282.1
• Coordinates: 65075..65305 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8C11_RS00350	59830..60618	+	789	WP_001206315.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-
A8C11_RS00355	60678..61502	+	825	WP_000722315.1	oxacillin-hydrolyzing class D beta-lactamase OXA-9	-
A8C11_RS00360	61702..62019	+	318	Protein_73	recombinase family protein	-
A8C11_RS00365	62202..63062	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
A8C11_RS00370	63147..63844	-	698	Protein_75	IS1 family transposase	-
A8C11_RS00380	63878..64588	-	711	WP_004152334.1	ATP-binding protein	-
A8C11_RS00385	64662..65078	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
A8C11_RS00390	65075..65305	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A8C11_RS00395	65262..65723	+	462	WP_072202616.1	hypothetical protein	-
A8C11_RS00400	65867..66217	+	351	WP_000493378.1	hypothetical protein	-
A8C11_RS00405	66268..67011	+	744	WP_000129823.1	hypothetical protein	-
A8C11_RS00410	67008..67784	+	777	WP_000015958.1	site-specific integrase	-
A8C11_RS00415	67842..68099	-	258	WP_000764642.1	hypothetical protein	-
A8C11_RS31305	68228..68332	-	105	WP_032409716.1	hypothetical protein	-
A8C11_RS00425	68867..69733	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / sul1 / qacE / cmlA1 / ant(3'')-Ia / ere(A) / ARR-3 / blaSHV-187 / aac(3)-IId / blaCTX-M-15 / aac(6')-Ib / blaOXA-9 / blaTEM-1A	-	1..70830	70830

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T65445 WP_001044770.1 NZ_CP016919:c65078-64662 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T65445 NZ_CP016919:c65078-64662 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT65445 WP_001261282.1 NZ_CP016919:c65305-65075 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT65445 NZ_CP016919:c65305-65075 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3