Detailed information of TA system TA065443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 276232..276926
• Replicon: chromosome
• Accession: NZ_CP016916
• Organism: Parageobacillus thermoglucosidasius strain TM242

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A369X1P3
• Locus tag: BCV54_RS01425
• Protein ID: WP_042384676.1
• Coordinates: 276232..276618 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A369X4Q0
• Locus tag: BCV54_RS01430
• Protein ID: WP_042384678.1
• Coordinates: 276618..276926 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV54_RS01400	271980..272663	+	684	WP_042384671.1	glycosyltransferase family 2 protein	-
BCV54_RS01405	272683..273051	+	369	WP_042384673.1	DUF2304 domain-containing protein	-
BCV54_RS01410	273038..273406	+	369	WP_041270157.1	EamA family transporter	-
BCV54_RS01415	273423..274688	+	1266	WP_042384675.1	glycosyltransferase family 39 protein	-
BCV54_RS01420	275150..276040	-	891	WP_042384703.1	ISNCY family transposase	-
BCV54_RS01425	276232..276618	-	387	WP_042384676.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCV54_RS01430	276618..276926	-	309	WP_042384678.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BCV54_RS01435	277577..278047	+	471	WP_003247869.1	transposase family protein	-
BCV54_RS20690	278184..278336	+	153	WP_153017391.1	hypothetical protein	-
BCV54_RS01440	278382..279104	-	723	WP_080695333.1	IS21-like element helper ATPase IstB	-
BCV54_RS01445	279105..280610	-	1506	WP_042384708.1	IS21 family transposase	-
BCV54_RS01450	280796..281035	+	240	WP_035501703.1	hypothetical protein	-
BCV54_RS20695	281245..281350	+	106	Protein_280	helix-turn-helix domain-containing protein	-
BCV54_RS01455	281392..281706	+	315	WP_072000118.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-116)

Antitoxin

• Antitoxin PemI-like (23-100)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14285.31 Da
• Isoelectric Point: 4.6190

>T65443 WP_042384676.1 NZ_CP016916:c276618-276232 [Parageobacillus thermoglucosidasius]
MPADVPERGDFVVLNFNPQAGHEQAGRRNAIVLSPKEFNQATGFIAVCPIINQKKGYPFEVDLPKEGISPDGDGYPITGV
VLTDQIKSLDWKARNLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Nucleotide

• Length: 387 bp

>T65443 NZ_CP016916:c276618-276232 [Parageobacillus thermoglucosidasius]
ATGCCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCAATCCACAAGCTGGTCATGAGCAGGCTGGGAG
AAGGAATGCTATTGTTTTATCGCCTAAAGAATTCAATCAAGCAACAGGATTTATAGCTGTTTGTCCGATTATCAATCAAA
AGAAGGGTTATCCCTTCGAAGTGGATTTACCAAAAGAAGGAATATCCCCTGATGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACTGATCAAATTAAATCATTGGATTGGAAAGCTCGTAATCTAAAAATTTTAAAAAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAAAATCATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11578.31 Da
• Isoelectric Point: 8.4665

>AT65443 WP_042384678.1 NZ_CP016916:c276926-276618 [Parageobacillus thermoglucosidasius]
MYSKEHLENKINQSKGVGYMSTVIVQKWGNSLGIRIPKDAADKIGIKQGSEIELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Nucleotide

• Length: 309 bp

>AT65443 NZ_CP016916:c276926-276618 [Parageobacillus thermoglucosidasius]
ATGTATTCAAAAGAACATTTAGAAAACAAGATAAACCAGAGTAAAGGGGTTGGCTACATGTCTACAGTTATTGTTCAAAA
ATGGGGAAACAGTTTGGGCATTCGTATCCCAAAAGATGCTGCTGATAAAATAGGGATTAAACAAGGTTCCGAAATAGAAT
TAAATGTGATTGGAAATGAAGGTATCATCACATTAAAGCCAAAAAGAACACGGAAGAAATACACACTGGAAGAACTAATA
TCACAGATTACACCTGAAAATCGACACGAGGAAATTGATTTCGGGATAGAAGGGCGTGAATTGATTTAA

Structures

Toxin

• AlphaFold DB: A0A369X1P3

Antitoxin

• AlphaFold DB: A0A369X4Q0