Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 3593146..3594732 Replicon chromosome
Accession NZ_CP016914
Organism Ralstonia solanacearum strain CQPS-1

Toxin (Protein)


Gene name hipA Uniprot ID A0A0S4V639
Locus tag BC350_RS16965 Protein ID WP_071507716.1
Coordinates 3593395..3594732 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag BC350_RS16960 Protein ID WP_028853251.1
Coordinates 3593146..3593391 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC350_RS16930 3589210..3590136 - 927 WP_028860692.1 muramoyltetrapeptide carboxypeptidase -
BC350_RS16935 3590133..3590684 - 552 WP_028860693.1 tRNA adenosine(34) deaminase TadA -
BC350_RS16940 3590704..3591180 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
BC350_RS16945 3591248..3591886 - 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
BC350_RS16955 3592203..3593042 + 840 WP_011001397.1 oxaloacetate decarboxylase -
BC350_RS16960 3593146..3593391 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
BC350_RS16965 3593395..3594732 + 1338 WP_071507716.1 type II toxin-antitoxin system HipA family toxin Toxin
BC350_RS16970 3594729..3595931 + 1203 WP_071507715.1 MFS transporter -
BC350_RS16975 3596157..3596885 + 729 WP_011001393.1 DsbA family protein -
BC350_RS16980 3596908..3597471 - 564 WP_071654081.1 RNA 2',3'-cyclic phosphodiesterase -
BC350_RS16985 3597652..3598350 - 699 WP_071507713.1 protocatechuate 3,4-dioxygenase subunit alpha -
BC350_RS16990 3598347..3599087 - 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48738.78 Da        Isoelectric Point: 6.7232

>T65441 WP_071507716.1 NZ_CP016914:3593395-3594732 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T65441 NZ_CP016914:3593395-3594732 [Ralstonia solanacearum]
ATGGCGCATCCCGAACTGCATCTGTGGATGAACGGCGAGCATGTCGGCATCTGGACCGCCAGCCGCGCCGGCGTGCCGAT
CCTGCGCTATGACGACGCCTGGCTGCGCTCGCCCAACATGCGGCCGCTGTCGCTGTCGCTGCCGATCCCGGCCGGTGTGC
CCGAGCTGCGCGGCCAGGTGGTCGACGACTACTTCGACAACCTGCTGCCCGAGGCCCAGCCCATCCGCGAGCGCGTGCGG
CGCCGCTTCGGCCTCGCCTCCACAGATGCCGCCGAATTGCTGGCCGCCATCGGCCGCGACTGCGTGGGCGCCGTGCAACT
GCTGCCGCCCGGCATGGCCCCCACCGGCTACGACCGCATCGACAGCGAGCCGCTCACCGACAGCCAGGTCGACACGCTGC
TGCGCAACCTCACCGCCACGCCCGCGCTGGGCGAGGACGCCGGCGACCCCGACGCCTTCCGCATCTCCATTGCCGGCGCG
CAGGAGAAAACCGCCCTGCTGCGCATCGGCGATGCCTGGCACCGGCCGCTGGGCGCCACGCCCACCACGCACATCCTCAA
GCTGCCGCTGGGGCTGATCGGCAACATCCGCGCCGACATGCAGGACTCGGTCGACAACGAATGGCTGTGCGCGCAGCTGC
TGGCGGCCTGGGGGCTGGAGGTGGCGCGCACCGAGATCGCCCGCTTCGGCGCCAGCAAAGTGCTGGCGGTCGAGCGCTTC
GACCGCCGCTGGATGCCGGGCAACGCCTGGATCGCGCGCCTGCCGCAGGAAGACTTCTGCCAGGCCACCGGCACGCCCGC
GCACCGCAAATACGAAAACGACGGCGGCCCCGGCATGCGCGCCTGCCTCGACCTGCTGTCCGCGTCCGAACACGCCATCG
ACGACAAGCGCCGCTTCGTGCTGGCGCAACTTGCCTTCTGGCTGCTGGCCGCCACCGACGGGCACGCGAAGAACTTCTCG
CTGGCGATCGGGCGCGGCGGGCGCTTCCGCATGACGCCGCTGTATGACGTGCTCTCGGTCTGGCCGATCATGGGCGACGG
GCCGAACCAGCTGTCGCCCTATCGCGCGCGGCTGGCGATGGCCCTGCGCGGCAAGAACGCGCATGCGCGGCTGGCCGACA
TCCACGTGCGCCACTGGCAGGCGCTGGCGGCGCAATCCGGCGTGCCGGACGCGTTCGCCGGCATGCAGGCGCTGGTCGAG
CGCGTGCCGGCGGGTCTCGAGCGGGTCGAGGCGATCCTGCCGCCCGACTTCTCGGGCCGGGTCTGGAGCAGCGTGCGCAC
CGGCGTGCTCGCGCAGGCCGAGCGCTTTCTCAACGAACTGGCGTCCGTCACCCCATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT65441 WP_028853251.1 NZ_CP016914:3593146-3593391 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT65441 NZ_CP016914:3593146-3593391 [Ralstonia solanacearum]
ATGGAGTCCCTGCTCTCCTCGGCGCCGCAGCTGTCGGCGCATCTGAAATCGTTGCGCAAGGCGCGCGGACTCACGCAGGC
CCAGTTGGGCGCACTGCTGGGCGTCAGCCAGAACCGCATCGCCGACATCGAAAAAGACCCGGGCGCGGTCGGCTTCGAGC
AGTTGCTGCGCATCCTGCACGCGCTCGGCGCGCAGCTGACGCTGCGCACGTCCGCGCCCGCCGCCGCCGCGCCCGCCGAC
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4V639

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References