Detailed information of TA system TA065438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1219575..1220491
• Replicon: chromosome
• Accession: NZ_CP016913
• Organism: Bacillus amyloliquefaciens strain RD7-7

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BARD7_RS06070
• Protein ID: WP_065981257.1
• Coordinates: 1219745..1220491 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BARD7_RS06065
• Protein ID: WP_003154807.1
• Coordinates: 1219575..1219745 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BARD7_RS06035	1216441..1216821	+	381	WP_065981254.1	hypothetical protein	-
BARD7_RS06040	1216826..1217023	+	198	WP_013351962.1	XkdX family protein	-
BARD7_RS06045	1217071..1217832	+	762	WP_065981255.1	phage portal protein	-
BARD7_RS06050	1217886..1218149	+	264	WP_013351964.1	hemolysin XhlA family protein	-
BARD7_RS06055	1218163..1218426	+	264	WP_013351965.1	phage holin	-
BARD7_RS06060	1218440..1219318	+	879	WP_065981256.1	N-acetylmuramoyl-L-alanine amidase	-
BARD7_RS06065	1219575..1219745	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BARD7_RS06070	1219745..1220491	-	747	WP_065981257.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BARD7_RS06075	1220599..1221597	-	999	WP_065981258.1	inorganic phosphate transporter	-
BARD7_RS06080	1221610..1222227	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BARD7_RS06085	1222511..1223827	-	1317	WP_065981259.1	amino acid permease	-
BARD7_RS06090	1224151..1225101	+	951	WP_065981260.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29041.53 Da
• Isoelectric Point: 4.6839

>T65438 WP_065981257.1 NZ_CP016913:c1220491-1219745 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQTRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T65438 NZ_CP016913:c1220491-1219745 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTATCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAAGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACAAGGGAAATATTGGAAGAAAATAA
TGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGACCCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT65438 WP_003154807.1 NZ_CP016913:c1219745-1219575 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT65438 NZ_CP016913:c1219745-1219575 [Bacillus amyloliquefaciens]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
CATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14