Detailed information of TA system TA065437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 508219..508856
• Replicon: chromosome
• Accession: NZ_CP016913
• Organism: Bacillus amyloliquefaciens strain RD7-7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BARD7_RS02395
• Protein ID: WP_003156187.1
• Coordinates: 508506..508856 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BARD7_RS02390
• Protein ID: WP_003156188.1
• Coordinates: 508219..508500 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BARD7_RS02370	504579..505178	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
BARD7_RS02375	505271..505636	+	366	WP_013351093.1	holo-ACP synthase	-
BARD7_RS18835	506043..506108	+	66	Protein_434	hypothetical protein	-
BARD7_RS02385	506931..508100	+	1170	WP_065980768.1	alanine racemase	-
BARD7_RS02390	508219..508500	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BARD7_RS02395	508506..508856	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BARD7_RS02400	508977..509798	+	822	WP_065980769.1	STAS domain-containing protein	-
BARD7_RS02405	509803..510168	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BARD7_RS02410	510171..510572	+	402	WP_065980770.1	anti-sigma regulatory factor	-
BARD7_RS02415	510584..511591	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
BARD7_RS02420	511655..511984	+	330	WP_065980771.1	anti-sigma factor antagonist	-
BARD7_RS02425	511981..512463	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
BARD7_RS02430	512429..513217	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
BARD7_RS02435	513217..513819	+	603	WP_065980772.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T65437 WP_003156187.1 NZ_CP016913:508506-508856 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T65437 NZ_CP016913:508506-508856 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCCACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT65437 WP_003156188.1 NZ_CP016913:508219-508500 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT65437 NZ_CP016913:508219-508500 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5