Detailed information of TA system TA065431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 573093..573649
• Replicon: chromosome
• Accession: NZ_CP016911
• Organism: Burkholderia pseudomallei strain Burk178-Type2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BFR06_RS03065
• Protein ID: WP_024429387.1
• Coordinates: 573359..573649 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M4UBZ0
• Locus tag: BFR06_RS03060
• Protein ID: WP_004541313.1
• Coordinates: 573093..573371 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFR06_RS39640	568611..569891	+	1281	WP_144399190.1	hypothetical protein	-
BFR06_RS03045	569938..570228	-	291	WP_071897845.1	hypothetical protein	-
BFR06_RS03050	570717..570986	+	270	WP_020850841.1	ribbon-helix-helix protein, CopG family	-
BFR06_RS03055	570989..573013	+	2025	WP_075018821.1	relaxase/mobilization nuclease domain-containing protein	-
BFR06_RS03060	573093..573371	+	279	WP_004541313.1	CopG family ribbon-helix-helix protein	Antitoxin
BFR06_RS03065	573359..573649	+	291	WP_024429387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFR06_RS03070	573652..573966	+	315	WP_023872355.1	hypothetical protein	-
BFR06_RS39645	574162..574410	+	249	WP_080251141.1	hypothetical protein	-
BFR06_RS40425	574515..575174	+	660	WP_024429389.1	hypothetical protein	-
BFR06_RS03080	575216..577420	-	2205	WP_024429390.1	AAA family ATPase	-
BFR06_RS03085	577647..578168	-	522	WP_024429391.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	560531..582515	21984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10906.47 Da
• Isoelectric Point: 9.5554

>T65431 WP_024429387.1 NZ_CP016911:573359-573649 [Burkholderia pseudomallei]
MPQVIFAPAAIGDMQRLREFLRPKNPDAARRAGEAIRRGVQALGAHPRMGRLIEDLPEQYREWLIDFGDSGYVARYRLDG
DTVTILAVRHQKEAGY

Nucleotide

• Length: 291 bp

>T65431 NZ_CP016911:573359-573649 [Burkholderia pseudomallei]
ATGCCACAAGTAATCTTTGCGCCGGCGGCGATCGGGGACATGCAACGGCTCCGCGAATTCCTGAGGCCGAAGAATCCCGA
TGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGTCCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAG
AAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATTTTGGGGATAGTGGCTATGTGGCTCGGTACCGCCTCGATGGC
GATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAAGCAGGGTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10810.11 Da
• Isoelectric Point: 4.9736

>AT65431 WP_004541313.1 NZ_CP016911:573093-573371 [Burkholderia pseudomallei]
MVTATSIKLDDELKGRVQHLAEARRRTPHWIMREAIEQYVEREEKREALNRDTLKAWDEFQATGLHVTAEEVDKWLASWG
TDDELPPPECHK

Nucleotide

• Length: 279 bp

>AT65431 NZ_CP016911:573093-573371 [Burkholderia pseudomallei]
ATGGTTACGGCAACATCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCACTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGTGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: M4UBZ0