Detailed information of TA system TA065429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1475022..1475677
• Replicon: chromosome
• Accession: NZ_CP016910
• Organism: Burkholderia pseudomallei strain Burk178-Type1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BFR05_RS28210
• Protein ID: WP_004521942.1
• Coordinates: 1475022..1475318 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: BFR05_RS28215
• Protein ID: WP_004202809.1
• Coordinates: 1475321..1475677 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFR05_RS28195	1470026..1471390	+	1365	WP_051781513.1	GGDEF domain-containing protein	-
BFR05_RS28200	1471501..1473750	+	2250	WP_009979045.1	glycosyltransferase	-
BFR05_RS28205	1473677..1474390	+	714	WP_080002914.1	cellulose biosynthesis protein BcsS	-
BFR05_RS28210	1475022..1475318	+	297	WP_004521942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFR05_RS28215	1475321..1475677	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
BFR05_RS28220	1475721..1476776	-	1056	WP_038789201.1	phage portal protein	-
BFR05_RS28225	1476773..1478542	-	1770	WP_024429661.1	terminase ATPase subunit family protein	-
BFR05_RS28230	1478686..1479495	+	810	WP_015985028.1	GPO family capsid scaffolding protein	-
BFR05_RS28235	1479529..1480542	+	1014	WP_024429662.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1460042..1500204	40162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10902.68 Da
• Isoelectric Point: 9.9369

>T65429 WP_004521942.1 NZ_CP016910:1475022-1475318 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIADELED

Nucleotide

• Length: 297 bp

>T65429 NZ_CP016910:1475022-1475318 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGACGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT65429 WP_004202809.1 NZ_CP016910:1475321-1475677 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT65429 NZ_CP016910:1475321-1475677 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3