65426

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2604875..2605412 Replicon chromosome
Accession NZ_CP016909
Organism Burkholderia pseudomallei strain Burk178-Type1

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BFR05_RS13485 Protein ID WP_004549731.1
Coordinates 2605143..2605412 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BFR05_RS13480 Protein ID WP_004525420.1
Coordinates 2604875..2605159 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFR05_RS39790 2600162..2600347 - 186 WP_024429283.1 hypothetical protein -
BFR05_RS13455 2600344..2602059 - 1716 WP_024429282.1 terminase large subunit -
BFR05_RS13460 2602063..2602539 - 477 WP_004549538.1 phage terminase small subunit P27 family -
BFR05_RS13465 2602677..2603033 - 357 WP_024429281.1 HNH endonuclease -
BFR05_RS13470 2603122..2603700 - 579 WP_038738294.1 hypothetical protein -
BFR05_RS13475 2603702..2604613 - 912 WP_143281104.1 hypothetical protein -
BFR05_RS13480 2604875..2605159 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BFR05_RS13485 2605143..2605412 - 270 WP_004549731.1 BrnT family toxin Toxin
BFR05_RS13490 2605461..2605904 - 444 WP_004548453.1 hypothetical protein -
BFR05_RS13495 2605901..2606161 - 261 WP_024429361.1 hypothetical protein -
BFR05_RS13500 2606137..2606469 - 333 WP_038725178.1 hypothetical protein -
BFR05_RS13505 2606499..2606897 - 399 WP_038738296.1 DUF1364 family protein -
BFR05_RS13510 2606912..2607259 - 348 WP_043289604.1 DUF2591 family protein -
BFR05_RS13515 2607256..2607600 - 345 WP_143297881.1 winged helix-turn-helix domain-containing protein -
BFR05_RS13520 2607610..2608146 - 537 WP_024429363.1 DUF1064 domain-containing protein -
BFR05_RS13525 2608143..2609135 - 993 WP_038738301.1 hypothetical protein -
BFR05_RS13530 2609289..2609549 - 261 WP_004526650.1 helix-turn-helix domain-containing protein -
BFR05_RS13535 2609546..2610367 - 822 WP_015967391.1 chromosome partitioning protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2559994..2628630 68636


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T65426 WP_004549731.1 NZ_CP016909:c2605412-2605143 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T65426 NZ_CP016909:c2605412-2605143 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT65426 WP_004525420.1 NZ_CP016909:c2605159-2604875 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT65426 NZ_CP016909:c2605159-2604875 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References