Detailed information of TA system TA065425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 573164..573720
• Replicon: chromosome
• Accession: NZ_CP016909
• Organism: Burkholderia pseudomallei strain Burk178-Type1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BFR05_RS03070
• Protein ID: WP_024429387.1
• Coordinates: 573430..573720 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M4UBZ0
• Locus tag: BFR05_RS03065
• Protein ID: WP_004541313.1
• Coordinates: 573164..573442 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFR05_RS39615	568682..569962	+	1281	WP_144399190.1	hypothetical protein	-
BFR05_RS03050	570009..570299	-	291	WP_071897845.1	hypothetical protein	-
BFR05_RS03055	570788..571057	+	270	WP_020850841.1	ribbon-helix-helix protein, CopG family	-
BFR05_RS03060	571060..573084	+	2025	WP_075018821.1	relaxase/mobilization nuclease domain-containing protein	-
BFR05_RS03065	573164..573442	+	279	WP_004541313.1	CopG family ribbon-helix-helix protein	Antitoxin
BFR05_RS03070	573430..573720	+	291	WP_024429387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFR05_RS03075	573723..574037	+	315	WP_023872355.1	hypothetical protein	-
BFR05_RS39620	574233..574481	+	249	WP_080251141.1	hypothetical protein	-
BFR05_RS40395	574586..575245	+	660	WP_024429389.1	hypothetical protein	-
BFR05_RS03085	575287..577491	-	2205	WP_024429390.1	AAA family ATPase	-
BFR05_RS03090	577718..578239	-	522	WP_024429391.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	560602..582586	21984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10906.47 Da
• Isoelectric Point: 9.5554

>T65425 WP_024429387.1 NZ_CP016909:573430-573720 [Burkholderia pseudomallei]
MPQVIFAPAAIGDMQRLREFLRPKNPDAARRAGEAIRRGVQALGAHPRMGRLIEDLPEQYREWLIDFGDSGYVARYRLDG
DTVTILAVRHQKEAGY

Nucleotide

• Length: 291 bp

>T65425 NZ_CP016909:573430-573720 [Burkholderia pseudomallei]
ATGCCACAAGTAATCTTTGCGCCGGCGGCGATCGGGGACATGCAACGGCTCCGCGAATTCCTGAGGCCGAAGAATCCCGA
TGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGTCCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAG
AAGATCTGCCTGAACAGTATCGGGAGTGGCTTATCGATTTTGGGGATAGTGGCTATGTGGCTCGGTACCGCCTCGATGGC
GATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAAGCAGGGTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10810.11 Da
• Isoelectric Point: 4.9736

>AT65425 WP_004541313.1 NZ_CP016909:573164-573442 [Burkholderia pseudomallei]
MVTATSIKLDDELKGRVQHLAEARRRTPHWIMREAIEQYVEREEKREALNRDTLKAWDEFQATGLHVTAEEVDKWLASWG
TDDELPPPECHK

Nucleotide

• Length: 279 bp

>AT65425 NZ_CP016909:573164-573442 [Burkholderia pseudomallei]
ATGGTTACGGCAACATCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCCGAGGCGCGCCGGCGTAC
CCCGCACTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGTGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCACGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: M4UBZ0