65405

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1806627..1807254 Replicon chromosome
Accession NZ_CP016893
Organism Thermoanaerobacterium thermosaccharolyticum strain TG57

Toxin (Protein)


Gene name mazF Uniprot ID D9TST7
Locus tag Thert_RS08960 Protein ID WP_013297077.1
Coordinates 1806904..1807254 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I3VUF9
Locus tag Thert_RS08955 Protein ID WP_013297076.1
Coordinates 1806627..1806902 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Thert_RS08935 1802394..1802756 + 363 WP_013297072.1 hypothetical protein -
Thert_RS08940 1802836..1804362 + 1527 WP_013297073.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
Thert_RS08945 1804376..1805359 + 984 WP_094043347.1 outer membrane lipoprotein carrier protein LolA -
Thert_RS08950 1805373..1806539 + 1167 WP_094397548.1 alanine racemase -
Thert_RS08955 1806627..1806902 + 276 WP_013297076.1 ribbon-helix-helix protein, CopG family Antitoxin
Thert_RS08960 1806904..1807254 + 351 WP_013297077.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
Thert_RS08965 1807371..1807820 + 450 WP_094043348.1 hypothetical protein -
Thert_RS08970 1807939..1809960 + 2022 WP_094397549.1 hypothetical protein -
Thert_RS08975 1810007..1811077 + 1071 WP_094397550.1 polysaccharide pyruvyl transferase CsaB -
Thert_RS08980 1811090..1811815 + 726 WP_094043354.1 WecB/TagA/CpsF family glycosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12945.03 Da        Isoelectric Point: 6.4714

>T65405 WP_013297077.1 NZ_CP016893:1806904-1807254 [Thermoanaerobacterium thermosaccharolyticum]
MIIKRGDILYADLSPVIGSEQGGVRPVLVIQNDIGNKYSPTVIVSAITSQINKAKLPTHVEINGTEYGLNKDSVILLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T65405 NZ_CP016893:1806904-1807254 [Thermoanaerobacterium thermosaccharolyticum]
ATGATTATAAAGCGTGGAGATATTTTATATGCTGATTTAAGCCCTGTGATAGGCTCAGAACAGGGTGGTGTTAGGCCCGT
ACTTGTTATACAAAACGACATCGGCAATAAGTACAGCCCTACAGTTATTGTATCAGCAATAACATCTCAAATAAACAAGG
CTAAATTGCCAACACATGTTGAGATAAATGGGACGGAATACGGACTTAATAAAGATTCTGTTATTTTGTTGGAACAAATT
AGGACTATAGATAAAAAGCGGCTTAGGGAAAAAATAGGTCATTTTGATCAAGAGATGATGGAAAAAGTAAATGAGGCGCT
GCAAATAAGCCTAGGGTTAATAGATTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10514.31 Da        Isoelectric Point: 7.7813

>AT65405 WP_013297076.1 NZ_CP016893:1806627-1806902 [Thermoanaerobacterium thermosaccharolyticum]
VGETKRILVSLPESLLEEVDVLAAMENRNRSEFIREAMKLYIKERKKMKIRESMKKGYLEMAAINAELAELGLTADNECF
NGYEKKLKKCD

Download         Length: 276 bp

>AT65405 NZ_CP016893:1806627-1806902 [Thermoanaerobacterium thermosaccharolyticum]
GTGGGCGAGACAAAAAGAATACTCGTCAGTTTGCCGGAGAGCTTACTGGAAGAAGTTGATGTGCTTGCTGCTATGGAAAA
TAGAAACCGCAGTGAATTTATAAGAGAAGCTATGAAGCTGTACATCAAGGAAAGAAAGAAGATGAAAATAAGGGAGAGCA
TGAAAAAAGGGTACCTTGAGATGGCGGCAATAAATGCAGAACTTGCGGAATTGGGCCTAACCGCTGACAATGAATGTTTT
AACGGATATGAAAAAAAATTAAAAAAGTGTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231VMD9

Antitoxin

Source ID Structure
AlphaFold DB I3VUF9

References