Detailed information of TA system TA065404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 35464..35990
• Replicon: plasmid unnamed3
• Accession: NZ_CP016892
• Organism: Pantoea agglomerans strain C410P1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BEE12_RS23090
• Protein ID: WP_069027133.1
• Coordinates: 35703..35990 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BEE12_RS23085
• Protein ID: WP_069027132.1
• Coordinates: 35464..35703 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE12_RS23070	31458..31979	+	522	WP_081273774.1	hypothetical protein	-
BEE12_RS23075	32029..33045	-	1017	WP_039387390.1	NAD(P)-dependent alcohol dehydrogenase	-
BEE12_RS23080	33150..35123	-	1974	WP_069027131.1	FIST C-terminal domain-containing protein	-
BEE12_RS23085	35464..35703	+	240	WP_069027132.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BEE12_RS23090	35703..35990	+	288	WP_069027133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEE12_RS23095	36035..36331	-	297	WP_039387382.1	hypothetical protein	-
BEE12_RS24450	36533..36709	+	177	WP_010257023.1	stress-induced protein	-
BEE12_RS23100	36773..37669	-	897	WP_069027134.1	LysR family transcriptional regulator	-
BEE12_RS23105	37803..38996	+	1194	WP_069027135.1	MFS transporter	-
BEE12_RS23110	39065..40105	+	1041	WP_069027136.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..173592	173592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-75)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11053.99 Da
• Isoelectric Point: 10.2591

>T65404 WP_069027133.1 NZ_CP016892:35703-35990 [Pantoea agglomerans]
MPWQLECDQRALKEWRKLGPTVREQLKKKLAECLESPRIEANKLSRMPDCYKIKLKSSGYRLAYQVIDDRVVVFVVAVGK
RERSEICSAASRRLS

Nucleotide

• Length: 288 bp

>T65404 NZ_CP016892:35703-35990 [Pantoea agglomerans]
ATGCCCTGGCAGCTTGAGTGTGATCAGAGGGCACTGAAAGAGTGGCGCAAGCTGGGCCCGACAGTACGTGAACAGCTTAA
AAAGAAGCTGGCTGAGTGTCTTGAGTCACCCAGAATTGAGGCGAACAAACTCAGCAGGATGCCTGACTGCTATAAGATCA
AGCTAAAGTCATCCGGTTATCGACTTGCATACCAGGTTATTGATGACCGGGTGGTCGTTTTTGTGGTGGCTGTCGGGAAA
AGAGAGCGCTCAGAAATCTGTTCTGCCGCTTCCAGGCGTCTCTCTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8894.31 Da
• Isoelectric Point: 8.6146

>AT65404 WP_069027132.1 NZ_CP016892:35464-35703 [Pantoea agglomerans]
VGSISIRIDDDLKRRSFAALKKMGVTPSDALRLMLAYIADNERPPFKQTSLSDEDAALVDIVKERLRNPEPVRITLDKL

Nucleotide

• Length: 240 bp

>AT65404 NZ_CP016892:35464-35703 [Pantoea agglomerans]
GTGGGTTCAATTAGCATCCGAATTGATGATGATCTTAAGAGACGATCCTTTGCTGCGCTCAAAAAAATGGGTGTGACGCC
CTCTGACGCGCTGCGCCTTATGCTTGCATATATTGCAGATAATGAACGTCCTCCCTTTAAGCAGACATCACTCAGTGACG
AAGATGCGGCGTTAGTAGACATAGTGAAAGAACGCCTGCGAAATCCTGAGCCTGTCAGGATCACGCTGGACAAGCTTTAA