Detailed information of TA system TA065403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafQ-relB/YafQ-RelB
• Location: 70235..70768
• Replicon: plasmid unnamed2
• Accession: NZ_CP016891
• Organism: Pantoea agglomerans strain C410P1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: BEE12_RS22210
• Protein ID: WP_069027002.1
• Coordinates: 70235..70441 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BEE12_RS22215
• Protein ID: WP_069027003.1
• Coordinates: 70511..70768 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE12_RS22190	65770..66978	+	1209	WP_069026998.1	AAA family ATPase	-
BEE12_RS22195	66975..67961	+	987	WP_069026999.1	ParB/RepB/Spo0J family partition protein	-
BEE12_RS25135	68492..68821	+	330	WP_155763637.1	hypothetical protein	-
BEE12_RS22205	68948..69772	-	825	WP_069027001.1	tyrosine-type recombinase/integrase	-
BEE12_RS22210	70235..70441	-	207	WP_069027002.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BEE12_RS22215	70511..70768	-	258	WP_069027003.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BEE12_RS22220	71114..71920	-	807	WP_069027004.1	helix-turn-helix domain-containing protein	-
BEE12_RS22225	72106..72795	+	690	WP_069027005.1	3-oxoacid CoA-transferase subunit A	-
BEE12_RS22230	72806..73458	+	653	Protein_75	3-oxoacid CoA-transferase subunit B	-
BEE12_RS22235	73469..74671	+	1203	WP_069027006.1	3-oxoadipyl-CoA thiolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..216117	216117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-65)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-81)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 8011.97 Da
• Isoelectric Point: 5.5499

>T65403 WP_069027002.1 NZ_CP016891:c70441-70235 [Pantoea agglomerans]
MTKLKPLITLLVNEQEIPAEYDDHPLQGNWRGYRDAHKEGDWIVIYRVDGEDLRLARTGTHQNIFSNY

Nucleotide

• Length: 207 bp

>T65403 NZ_CP016891:c70441-70235 [Pantoea agglomerans]
ATGACCAAACTTAAACCGCTAATCACGTTACTGGTCAACGAACAGGAAATCCCTGCCGAATACGATGATCATCCTCTGCA
GGGTAACTGGCGCGGCTACCGCGATGCGCATAAGGAAGGCGACTGGATAGTGATTTATCGGGTTGATGGAGAGGATCTTC
GGCTTGCACGAACCGGAACACATCAGAACATTTTCAGCAATTATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9261.81 Da
• Isoelectric Point: 10.3838

>AT65403 WP_069027003.1 NZ_CP016891:c70768-70511 [Pantoea agglomerans]
MNADALVRARIPKDTKDRAVAALDKMGLSTSGVIRLVMMRVAEEGKLPFEVKTPNVATRRAMAQLKSGKGERFESADALF
EDLGI

Nucleotide

• Length: 258 bp

>AT65403 NZ_CP016891:c70768-70511 [Pantoea agglomerans]
ATGAACGCAGATGCACTGGTAAGAGCACGTATTCCAAAGGATACGAAGGACCGAGCCGTCGCTGCACTGGATAAGATGGG
CCTCAGCACATCGGGCGTTATCCGCCTGGTAATGATGCGTGTTGCAGAAGAAGGTAAACTGCCTTTTGAAGTAAAGACGC
CAAACGTTGCTACCCGCAGAGCTATGGCTCAGCTGAAAAGTGGCAAAGGCGAGCGTTTCGAAAGCGCTGATGCCCTGTTT
GAAGACTTAGGCATCTAA