Detailed information of TA system TA065399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3491712..3492375
• Replicon: chromosome
• Accession: NZ_CP016889
• Organism: Pantoea agglomerans strain C410P1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BEE12_RS15990
• Protein ID: WP_069026199.1
• Coordinates: 3491712..3492026 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BEE12_RS15995
• Protein ID: WP_033757561.1
• Coordinates: 3492019..3492375 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE12_RS15970	3487134..3488531	+	1398	WP_069026196.1	class II fumarate hydratase	-
BEE12_RS15975	3488575..3489504	-	930	WP_069026197.1	DNA replication terminus site-binding protein	-
BEE12_RS15980	3489707..3490891	+	1185	WP_069026198.1	MFS transporter	-
BEE12_RS15985	3491099..3491593	+	495	WP_031593600.1	Rrf2 family transcriptional regulator	-
BEE12_RS15990	3491712..3492026	+	315	WP_069026199.1	type II toxin-antitoxin system HicA family toxin	Toxin
BEE12_RS15995	3492019..3492375	+	357	WP_033757561.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BEE12_RS16000	3492463..3495615	-	3153	WP_069026200.1	multidrug efflux RND transporter permease subunit OqxB	-
BEE12_RS16005	3495628..3496781	-	1154	Protein_3142	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (41-90)

Antitoxin

• Uncharacterised protein family HicB (59-111)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11834.52 Da
• Isoelectric Point: 8.7203

>T65399 WP_069026199.1 NZ_CP016889:3491712-3492026 [Pantoea agglomerans]
MTSLDEVAEVRMNTVVRDLRKKHRETLEQVFQMPPPSGLKWQAVESLIRALGGEIKEGSGSRVRFLLRGSIARFHRPHPS
PDTDRGAVVNLREWLESIGVDPYE

Nucleotide

• Length: 315 bp

>T65399 NZ_CP016889:3491712-3492026 [Pantoea agglomerans]
ATGACTTCACTGGATGAAGTCGCGGAGGTCAGGATGAACACCGTTGTCAGGGACTTACGGAAAAAACATCGTGAAACGTT
GGAGCAGGTTTTTCAAATGCCGCCGCCGTCCGGACTGAAATGGCAGGCAGTTGAGTCATTAATCAGGGCTCTGGGTGGTG
AGATTAAAGAAGGCAGTGGTTCACGGGTGCGTTTTCTTCTCAGAGGAAGCATAGCGCGTTTTCACCGGCCGCATCCTTCG
CCAGACACCGACAGAGGTGCAGTTGTTAATCTGCGTGAATGGCTGGAAAGTATTGGAGTCGATCCCTATGAATAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13250.86 Da
• Isoelectric Point: 4.8896

>AT65399 WP_033757561.1 NZ_CP016889:3492019-3492375 [Pantoea agglomerans]
MNNRSNSSITVAGQPAVITYVPELNMFRGRFTGLSGYCDFVSDSVQGLYKEGEISLREYLEDCAASGINPYAEQEKIKTF
TLRYPESLGERLSQAAAQNETSLNAFIIETLNERLKRR

Nucleotide

• Length: 357 bp

>AT65399 NZ_CP016889:3492019-3492375 [Pantoea agglomerans]
ATGAATAATCGTTCAAATTCATCAATCACCGTCGCCGGGCAACCCGCCGTGATAACTTATGTTCCTGAACTCAACATGTT
CCGTGGGCGCTTTACTGGCCTGTCAGGCTACTGTGATTTTGTCTCTGACAGCGTACAGGGGCTGTACAAAGAGGGCGAGA
TTTCGCTGCGCGAATACCTGGAAGATTGTGCAGCCTCCGGCATTAATCCCTATGCTGAGCAGGAAAAAATTAAGACCTTT
ACGCTGCGCTATCCTGAATCGCTGGGAGAGCGCCTGTCTCAGGCTGCCGCGCAAAATGAAACCTCGCTGAATGCGTTTAT
CATAGAAACGCTGAATGAAAGATTGAAGCGGCGCTAA