Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2587389..2588006 Replicon chromosome
Accession NZ_CP016889
Organism Pantoea agglomerans strain C410P1

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag BEE12_RS11675 Protein ID WP_003850458.1
Coordinates 2587389..2587604 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag BEE12_RS11680 Protein ID WP_003850455.1
Coordinates 2587629..2588006 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE12_RS11645 2583307..2583645 + 339 WP_003850469.1 P-II family nitrogen regulator -
BEE12_RS11650 2583680..2584966 + 1287 WP_010255877.1 ammonium transporter AmtB -
BEE12_RS11655 2585017..2585880 - 864 WP_010255880.1 acyl-CoA thioesterase II -
BEE12_RS11660 2586071..2586628 + 558 WP_010255881.1 YbaY family lipoprotein -
BEE12_RS11665 2586668..2586979 - 312 WP_031592174.1 MGMT family protein -
BEE12_RS11675 2587389..2587604 - 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
BEE12_RS11680 2587629..2588006 - 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
BEE12_RS11685 2588154..2588507 - 354 WP_069025895.1 hypothetical protein -
BEE12_RS11690 2588849..2589517 + 669 WP_069026576.1 ABC transporter ATP-binding protein -
BEE12_RS11695 2589514..2590353 + 840 WP_010255890.1 metal ABC transporter permease -
BEE12_RS11700 2590369..2591247 + 879 WP_069025896.1 metal ABC transporter substrate-binding protein -
BEE12_RS23895 2591293..2591433 - 141 WP_010255896.1 type B 50S ribosomal protein L36 -
BEE12_RS11705 2591445..2591699 - 255 WP_010671219.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T65398 WP_003850458.1 NZ_CP016889:c2587604-2587389 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T65398 NZ_CP016889:c2587604-2587389 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT65398 WP_003850455.1 NZ_CP016889:c2588006-2587629 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT65398 NZ_CP016889:c2588006-2587629 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGCCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References