Detailed information of TA system TA065397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2332056..2332687
• Replicon: chromosome
• Accession: NZ_CP016889
• Organism: Pantoea agglomerans strain C410P1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BEE12_RS23865
• Protein ID: WP_071941068.1
• Coordinates: 2332056..2332235 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BEE12_RS10470
• Protein ID: WP_069025829.1
• Coordinates: 2332262..2332687 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE12_RS10460	2328387..2330288	+	1902	WP_039386527.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
BEE12_RS10465	2330449..2331873	+	1425	WP_010253950.1	dihydrolipoyl dehydrogenase	-
BEE12_RS23865	2332056..2332235	+	180	WP_071941068.1	type II toxin-antitoxin system HicA family toxin	Toxin
BEE12_RS10470	2332262..2332687	+	426	WP_069025829.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BEE12_RS10475	2333398..2334297	-	900	WP_031593562.1	LysR family transcriptional regulator	-
BEE12_RS10480	2334403..2334912	+	510	WP_039391324.1	phenolic acid decarboxylase	-
BEE12_RS10485	2334999..2335856	+	858	WP_069025830.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6566.65 Da
• Isoelectric Point: 11.0668

>T65397 WP_071941068.1 NZ_CP016889:2332056-2332235 [Pantoea agglomerans]
MDSSTLISEMKADGWVLTRINGSHHHFTHPTKPGLVTVPHPKKDLPIGTVKSIRKQARI

Nucleotide

• Length: 180 bp

>T65397 NZ_CP016889:2332056-2332235 [Pantoea agglomerans]
ATGGATAGCAGCACGTTAATTTCCGAAATGAAGGCTGATGGCTGGGTTCTGACAAGGATAAACGGTAGCCATCACCATTT
CACACATCCGACAAAACCGGGGCTGGTTACAGTACCCCATCCTAAAAAAGACTTACCGATCGGTACGGTAAAGAGCATCA
GGAAACAAGCGAGAATTTAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15339.51 Da
• Isoelectric Point: 4.6233

>AT65397 WP_069025829.1 NZ_CP016889:2332262-2332687 [Pantoea agglomerans]
MFYPIAIEAGDHENAYGVIVPDLPGCFSAGDTLDEAIKNAKEAITGHIELCVELGHEIPAVSTIEALAANPDYQGYIWAL
VDVDVIRLLGGSEKINVTLPRSLIDRIDRCVASHPEYKSRSGFLAQVALERITAPINRPQK

Nucleotide

• Length: 426 bp

>AT65397 NZ_CP016889:2332262-2332687 [Pantoea agglomerans]
ATGTTTTATCCAATCGCGATTGAAGCGGGTGATCATGAAAACGCATACGGTGTAATCGTTCCCGATTTACCAGGGTGTTT
CTCTGCCGGGGATACTCTTGATGAAGCCATTAAAAACGCCAAAGAGGCTATCACAGGGCATATCGAATTATGTGTAGAGC
TCGGTCATGAGATCCCTGCCGTGTCCACCATTGAGGCTCTGGCAGCAAATCCTGACTATCAGGGTTATATCTGGGCATTG
GTTGATGTAGATGTCATTCGCTTGCTGGGCGGCTCTGAAAAAATCAACGTCACTTTACCCAGGTCGCTAATTGATCGGAT
TGATCGTTGTGTGGCTTCACACCCGGAATACAAAAGTCGCTCAGGCTTTCTGGCTCAGGTAGCGCTTGAGCGTATTACCG
CTCCGATTAACAGGCCTCAAAAGTAA