Detailed information of TA system TA065393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3834653..3835338
• Replicon: chromosome
• Accession: NZ_CP016888
• Organism: Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: BEE64_RS18050
• Protein ID: WP_003417998.1
• Coordinates: 3834928..3835338 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BEE64_RS18045
• Protein ID: WP_180895138.1
• Coordinates: 3834653..3834931 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE64_RS18025	3830642..3832243	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
BEE64_RS18030	3832260..3832964	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
BEE64_RS18035	3833082..3833648	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
BEE64_RS18040	3833710..3834597	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
BEE64_RS18045	3834653..3834931	+	279	WP_180895138.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BEE64_RS18050	3834928..3835338	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
BEE64_RS18055	3835371..3837107	-	1737	WP_003418002.1	cholesterol oxidase	-
BEE64_RS18060	3837163..3838290	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
BEE64_RS18065	3838310..3839899	-	1590	WP_003901630.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-58)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T65393 WP_003417998.1 NZ_CP016888:3834928-3835338 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T65393 NZ_CP016888:3834928-3835338 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10227.70 Da
• Isoelectric Point: 8.0222

>AT65393 WP_180895138.1 NZ_CP016888:3834653-3834931 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGCKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT65393 NZ_CP016888:3834653-3834931 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCTGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: G0TIR9