Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2867728..2868365 Replicon chromosome
Accession NZ_CP016888
Organism Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag BEE64_RS13475 Protein ID WP_003413180.1
Coordinates 2867728..2868123 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag BEE64_RS13480 Protein ID WP_003413183.1
Coordinates 2868120..2868365 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE64_RS13440 2863132..2864343 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BEE64_RS13445 2864470..2865129 + 660 WP_003902296.1 hypothetical protein -
BEE64_RS13450 2865126..2865788 + 663 WP_003905878.1 hypothetical protein -
BEE64_RS22230 2865785..2866062 + 278 Protein_2626 type II toxin-antitoxin system VapB family antitoxin -
BEE64_RS13455 2866155..2866568 + 414 WP_003413164.1 PIN domain nuclease -
BEE64_RS13460 2866607..2866864 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
BEE64_RS13465 2866861..2867238 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
BEE64_RS13470 2867254..2867628 - 375 WP_003413177.1 hypothetical protein -
BEE64_RS13475 2867728..2868123 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
BEE64_RS13480 2868120..2868365 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
BEE64_RS13485 2868776..2869195 - 420 WP_003413190.1 A24 family peptidase -
BEE64_RS13490 2869207..2870016 - 810 WP_003413193.1 shikimate dehydrogenase -
BEE64_RS13495 2870013..2871266 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BEE64_RS13500 2871259..2871771 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T65379 WP_003413180.1 NZ_CP016888:c2868123-2867728 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T65379 NZ_CP016888:c2868123-2867728 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT65379 WP_003413183.1 NZ_CP016888:c2868365-2868120 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT65379 NZ_CP016888:c2868365-2868120 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References