Detailed information of TA system TA065376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2852269..2852909
• Replicon: chromosome
• Accession: NZ_CP016888
• Organism: Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BEE64_RS13375
• Protein ID: WP_003412970.1
• Coordinates: 2852269..2852688 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BEE64_RS13380
• Protein ID: WP_003412975.1
• Coordinates: 2852685..2852909 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE64_RS13350	2847859..2848581	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
BEE64_RS13355	2849098..2849320	+	223	Protein_2605	type II toxin-antitoxin system VapB family antitoxin	-
BEE64_RS13360	2849317..2849718	+	402	WP_003412963.1	PIN domain-containing protein	-
BEE64_RS13365	2849753..2850673	-	921	WP_003412965.1	restriction endonuclease	-
BEE64_RS22610	2851014..2851259	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BEE64_RS13370	2851318..2852268	+	951	WP_003911916.1	Lsr2 family protein	-
BEE64_RS13375	2852269..2852688	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BEE64_RS13380	2852685..2852909	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BEE64_RS13385	2852940..2855783	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BEE64_RS13390	2855855..2856256	-	402	WP_003412981.1	hypothetical protein	-
BEE64_RS13395	2856256..2856726	-	471	WP_003899364.1	transcription antitermination factor NusB	-
BEE64_RS13400	2856729..2857292	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T65376 WP_003412970.1 NZ_CP016888:c2852688-2852269 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T65376 NZ_CP016888:c2852688-2852269 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT65376 WP_003412975.1 NZ_CP016888:c2852909-2852685 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT65376 NZ_CP016888:c2852909-2852685 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09